Four conductance levels of cloned cardiac L-type Ca2+channel α1and α1/β subunits
| Autor: | K Mannen, N Gondo, M Arita, Atsuko Yatani, S.A Green, K Ono |
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| Rok vydání: | 1998 |
| Předmět: |
Subconductance
Protein subunit Biophysics Biology Biochemistry Chinese hamster α Subunit Dihydropyridine Structural Biology β subunit Genetics medicine Fibroblast Molecular Biology Chinese hamster fibroblast Calcium channel Conductance Cell Biology biology.organism_classification Molecular biology β Subunit R-type calcium channel medicine.anatomical_structure medicine.drug |
| Zdroj: | FEBS Letters. 423:86-92 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(98)00070-2 |
| Popis: | Formation of single channel subconductance is one of the unique characteristics of the L-type Ca 2+ channel. Although α 1 subunit exhibits a primary function of the channel, it remains uncertain whether α 1 subunit alone is able to produce subconductance. We tested this by studying single channel currents of cloned cardiac α 1 subunit expressed in Chinese hamster fibroblast cells, with/without coexpression of cardiac β subunit. The α 1 subunit exhibited four distinct levels of conductance (22.7, 14.3, 6.2 and 3.2 pS). Coexpression of β subunit significantly increased the number of openings in all four levels of conductance without changing the conductance values. |
| Databáze: | OpenAIRE |
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