Heterogeneity in the expression of surface-exposed epitopes among larvae of Ascaris lumbricoides
| Autor: | Eleanor M. Fraser, Malcolm W. Kennedy |
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| Rok vydání: | 1991 |
| Předmět: |
animal structures
Immunology Antibodies Helminth Fluorescent Antibody Technique Immunofluorescence Epitope Epitopes Antigen Immunity medicine Animals Humans Antiserum Ascariasis Polymorphism Genetic medicine.diagnostic_test biology fungi Ascaris biology.organism_classification Antigens Helminth Humoral immunity Antigens Surface biology.protein Parasitology Rabbits Ascaris lumbricoides Antibody |
| Zdroj: | Parasite immunology. 13(2) |
| ISSN: | 0141-9838 |
| Popis: | Quantitative immunofluorescence was used to examine differences in the binding of antibody to the surfaces of individual living infective stage larvae of Ascaris lumbricoides. Using rabbit antisera, it was first established that larvae cultured for 48 h after artificial hatching were relatively uniform in their levels of antibody binding and in minimal exposure of epitopes expressed by later larval stages. Aliquots from a pool of larvae were probed with serum from individual infected people living in an endemic area of Nigeria. The larvae used were derived from parasites collected in the same geographical area in which serum donors were living. Two principal points emerged. First, serum donors varied considerably in the degree to which their antibody bound to the larvae. Secondly, the binding of antibody from a given donor revealed remarkable heterogeneity in surface epitope expression. Such intra-specific variability in antigen expression has considerable implications for the development of immunity to parasitic nematodes. |
| Databáze: | OpenAIRE |
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