Electrochemical aptasensor based on anisotropically modified (Janus-type) gold nanoparticles for determination of C-reactive protein

Autor: Anabel Villalonga, Alfredo Sánchez, Diana Vilela, Beatriz Mayol, Paloma Martínez-Ruíz, Reynaldo Villalonga
Rok vydání: 2022
Předmět:
Zdroj: Microchimica Acta. 189
ISSN: 1436-5073
0026-3672
DOI: 10.1007/s00604-022-05420-4
Popis: Novel Janus nanoparticles based on Au colloids anisotropically modified with polyamidoamine dendrons were prepared though a masking/toposelective modification approach. These nanomaterials were further functionalized with horseradish peroxidase on the dendritic face and provided on the opposite metal surface with a ssDNA aptamer for C-reactive protein (CRP). The resulting nanoparticles were employed as biorecognition/signaling elements to construct an amperometric aptasensor with sandwich-type architecture for the specific detection of this cardiac biomarker. To do this, screen-printed carbon electrodes modified with electrodeposited Au nanoparticles and functionalized with anti-CRP aptamers were used as transduction interface. The aptasensor was employed for the amperometric detection of CRP (working potential: - 200 mV vs pseudo-Ag/AgCl) in the broad range from 10 pg·mL
Databáze: OpenAIRE
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