Insemination with border disease virus-infected semen results in seroconversion in cows but not persistent infection in fetuses
| Autor: | Matthias Schweizer, Ueli Braun, Sandra Züblin, Monika Hilbe, Michèle von Büren, Reto Zanoni, Fredi Janett |
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| Přispěvatelé: | University of Zurich, Braun, Ueli |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
040301 veterinary sciences medicine.medical_treatment 3400 General Veterinary viruses animal diseases 10184 Institute of Veterinary Pathology Semen Persistent infection Insemination BDV 0403 veterinary science Border disease virus 03 medical and health sciences Medicine Seroconversion lcsh:Veterinary medicine biology General Veterinary 630 Agriculture business.industry Transmission (medicine) urogenital system Artificial insemination Pestivirus Border Disease 04 agricultural and veterinary sciences General Medicine biology.organism_classification Virology 10187 Department of Farm Animals 030104 developmental biology lcsh:SF600-1100 570 Life sciences Cattle business |
| Zdroj: | Braun, Ueli; Janett, Fredi; Züblin, Sandra; von Büren, Michèle; Hilbe, Monika; Zanoni, Reto Giacomo; Schweizer, Matthias (2018). Insemination with border disease virus-infected semen results in seroconversion in cows but not persistent infection in fetuses. BMC veterinary research, 14(1), pp. 1-8. BioMed Central 10.1186/s12917-018-1472-6 BMC Veterinary Research, Vol 14, Iss 1, Pp 1-8 (2018) |
| DOI: | 10.1186/s12917-018-1472-6 |
| Popis: | Background This study examined various health variables in cows after artificial insemination with Border disease virus (BDV)-infected semen and the occurrence of persistent infection in ensuing fetuses. Five cows were inseminated (day 0) with BDV-infected semen as well as with semen from a fertile Eringer bull. One cow, inseminated with virus-free semen only, served as a control. Clinical examination, assessment of eating and rumination activities, measurement of intraruminal temperature and leukocyte count were used to monitor the health of the cows. Blood samples were collected at regular intervals for the detection of viral RNA and antibodies against BDV, and the cows were slaughtered on day 56. The uteri, placentae and fetuses were examined macroscopically, histologically, immunohistochemically and by means of molecular methods for the presence of pestiviruses. Results The demeanour, eating and rumination activities and intraruminal temperature were not affected by insemination with BDV-infected semen, whereas the total leukocyte and lymphocyte counts dropped transiently and were significantly lower on day 6 than on day 0. Seroconversion occurred by day 28 in the five infected cows but not in the control cow. The uteri, placentae and fetuses had no macroscopic or histological lesions, and immunohistochemical examination and RT-PCR were negative for pestiviruses. Conclusions The findings showed that cows inseminated with BDV-infected semen seroconverted and fetuses thus produced were not persistently infected. Transmission of BDV to cattle through infected semen, therefore, seems to be of minor importance. |
| Databáze: | OpenAIRE |
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