Pyrosequencing assessment of prokaryotic and eukaryotic diversity in biofilm communities from a F rench river
| Autor: | Gwenaël Le Moal, Christiane Forestier, Gérard Coffe, Geneviève Bricheux, Jacques Bohatier, Damien Balestrino, Nicolas Charbonnel, Loïc Morin |
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| Přispěvatelé: | Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Centre National de la Recherche Scientifique (CNRS), Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Centre National de la Recherche Scientifique (CNRS)-Université d'Auvergne - Clermont-Ferrand I (UdA) |
| Rok vydání: | 2013 |
| Předmět: |
Biodiversity
DNA Ribosomal Microbiology biofilm 03 medical and health sciences Rivers 14. Life underwater Aquatic communities Bacterial phyla Physiological Phenomena Original Research 030304 developmental biology 0303 health sciences Bacteria biology pyrosequencing 030306 microbiology Ecology Phylum Verrucomicrobia Eukaryota Bacteroidetes Genes rRNA Sequence Analysis DNA 15. Life on land biology.organism_classification Archaea Biota 6. Clean water 13. Climate action Biofilms Pyrosequencing France [SDE.BE]Environmental Sciences/Biodiversity and Ecology Proteobacteria |
| Zdroj: | MicrobiologyOpen MicrobiologyOpen, Wiley, 2013, epub ahead of print. ⟨10.1002/mbo3.80⟩ MicrobiologyOpen, 2013, epub ahead of print. ⟨10.1002/mbo3.80⟩ |
| ISSN: | 2045-8827 |
| DOI: | 10.1002/mbo3.80 |
| Popis: | International audience; Despite the recent and significant increase in the study of aquatic microbial communities, little is known about the microbial diversity of complex ecosystems such as running waters. This study investigated the biodiversity of biofilm communities formed in a river with 454 Sequencing™. This river has the particularity of integrating both organic and microbiological pollution, as receiver of agricultural pollution in its upstream catchment area and urban pollution through discharges of the wastewater treatment plant of the town of Billom. Different regions of the small subunit (SSU) ribosomal RNA gene were targeted using nine pairs of primers, either universal or specific for bacteria, eukarya, or archaea. Our aim was to characterize the widest range of rDNA sequences using different sets of polymerase chain reaction (PCR) primers. A first look at reads abundance revealed that a large majority (47-48%) were rare sequences ( |
| Databáze: | OpenAIRE |
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