Modelling structures in cryo-EM maps
| Autor: | Matteo Dal Peraro, Sylvain Träger, Sony Malhotra, Maya Topf |
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Models
Molecular real-space refinement Cryo-electron microscopy Computer science density maps 03 medical and health sciences 0302 clinical medicine Structural Biology Atomic model atomic models Molecular Biology Prior information 030304 developmental biology Structure (mathematical logic) validation 0303 health sciences Resolution (electron density) Cryoelectron Microscopy Proteins resolution dynamics prediction initiation protein-structure Algorithm 030217 neurology & neurosurgery microscopy data |
| Popis: | Recent advances in structure determination of sub-cellular structures using cryo-electron microscopy and tomography have enabled us to understand their architecture in a more detailed manner and gain insight into their function. The choice of approach to use for atomic model building, fitting, refinement and validation in the 3D map resulting from these experiments depends primarily on the resolution of the map and the prior information on the corresponding model. Here, we survey some of such methods and approaches and highlight their uses in specific recent examples. |
| Databáze: | OpenAIRE |
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