A Crown-of-Thorns Seastar recombinant relaxin-like gonad-stimulating peptide triggers oocyte maturation and ovulation
| Autor: | Scott F. Cummins, Meaghan K. Smith, Hoang Dinh Chieu, Cherie A. Motti, Abigail Elizur, Joseph Aizen, Benjamin Mos |
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| Rok vydání: | 2019 |
| Předmět: |
Ovulation
Gonad media_common.quotation_subject Population 030209 endocrinology & metabolism Starfish 03 medical and health sciences 0302 clinical medicine Endocrinology medicine Animals education 030304 developmental biology media_common 0303 health sciences education.field_of_study Germinal vesicle biology Ovary Relaxin Crown of Thorns Acanthaster biology.organism_classification Oocyte Recombinant Proteins Cell biology medicine.anatomical_structure Echinoderm Oocytes Biological Assay Female Animal Science and Zoology |
| Zdroj: | General and Comparative Endocrinology. 281:41-48 |
| ISSN: | 0016-6480 |
| Popis: | The Acanthaster planci species-complex [Crown-of-Thorns Seastar (COTS)] are highly fecund echinoderms that exhibit population outbreaks on coral reef ecosystems worldwide, including the Australian Great Barrier Reef. A better understanding of the COTS molecular biology is critical towards efforts in controlling outbreaks and assisting reef recovery. In seastars, the heterodimeric relaxin-like gonad stimulating peptide (RGP) is responsible for triggering a neuroendocrine cascade that regulates resumption of oocyte meiosis prior to spawning. Our comparative RNA-seq analysis indicates a general increase in RGP gene expression in the female radial nerve cord during the reproductive season. Also, the sensory tentacles demonstrate a significantly higher expression level than radial nerve cord. A recombinant COTS RGP, generated in a yeast expression system, is highly effective in inducing oocyte germinal vesicle breakdown (GVBD), followed by ovulation from ovarian fragments. The findings of this study provide a foundation for more in-depth molecular analysis of the reproductive neuroendocrine physiology of the COTS and the RGP. |
| Databáze: | OpenAIRE |
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