Antibacterial and Antivirulence Activity of Glucocorticoid PYED-1 against Stenotrophomonas maltophilia
| Autor: | Adriana Vollaro, Raffaele Zarrilli, Eliana Esposito, Anna Esposito, Eliana De Gregorio, Annalisa Guaragna, Daniele D'Alonzo |
|---|---|
| Přispěvatelé: | Esposito, A., Vollaro, A., Esposito, E. P., D'Alonzo, D., Guaragna, A., Zarrilli, R., De Gregorio, E. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
congenital hereditary and neonatal diseases and abnormalities medicine.drug_class Antibiotics Virulence Biochemistry Microbiology Cystic fibrosis checkerboard assay medicine S. maltophilia Pharmacology (medical) quantitative real-time PCR General Pharmacology Toxicology and Pharmaceutics anti-virulence agent antimicrobial activity biology lcsh:RM1-950 Biofilm biochemical phenomena metabolism and nutrition medicine.disease biology.organism_classification Antimicrobial deflazacort (DFZ) S. maltophilia Stenotrophomonas maltophilia Infectious Diseases lcsh:Therapeutics. Pharmacology Deflazacort (DFZ) bacteria Antibacterial activity Bacteria |
| Zdroj: | Antibiotics Volume 9 Issue 3 Antibiotics, Vol 9, Iss 3, p 105 (2020) |
| ISSN: | 2079-6382 |
| DOI: | 10.3390/antibiotics9030105 |
| Popis: | Stenotrophomonas maltophilia, an environmental Gram-negative bacterium, is an emerging nosocomial opportunistic pathogen that causes life-threatening infections in immunocompromised patients and chronic pulmonary infections in cystic fibrosis patients. Due to increasing resistance to multiple classes of antibiotics, S. maltophilia infections are difficult to treat successfully. This makes the search for new antimicrobial strategies mandatory. In this study, the antibacterial activity of the heterocyclic corticosteroid deflazacort and several of its synthetic precursors was tested against S. maltophilia. All compounds were not active against standard strain S. maltophilia K279a. The compound PYED-1 (pregnadiene-11-hydroxy-16&alpha 17&alpha epoxy-3,20-dione-1) showed a weak effect against some S. maltophilia clinical isolates, but exhibited a synergistic effect with aminoglycosides. PYED-1 at sub-inhibitory concentrations decreased S. maltophilia biofilm formation. Quantitative real-time polymerase chain reaction (RT-qPCR) analysis demonstrated that the expression of biofilm- and virulence- associated genes (StmPr1, StmPr3, sphB, smeZ, bfmA, fsnR) was significantly suppressed after PYED-1 treatment. Interestingly, PYED-1 also repressed the expression of the genes aph (3´ )-IIc, aac (6´ )-Iz, and smeZ, involved in the resistance to aminoglycosides. |
| Databáze: | OpenAIRE |
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