Use of isoelectric focusing to define major histocompatibility complex class I polymorphism in goats
| Autor: | G Ruff, M. F. Sanders, Irma Joosten, E. J. Hensen |
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| Rok vydání: | 2009 |
| Předmět: |
Genetics
Polymorphism Genetic Isoelectric focusing Goats Histocompatibility Antigens Class I Haplotype Genes MHC Class I Locus (genetics) General Medicine Immunogenetics Biology Major histocompatibility complex Breed Haplotypes MHC class I biology.protein Animals Electrophoresis Polyacrylamide Gel Animal Science and Zoology Typing Isoelectric Focusing |
| Zdroj: | Animal Genetics. 24:47-51 |
| ISSN: | 1365-2052 0268-9146 |
| DOI: | 10.1111/j.1365-2052.1993.tb00918.x |
| Popis: | Summary We have used biochemical methods to extend and improve serological class I typing using a panel of 77 Swiss goats of the Saanen breed, comprising dam-offspring combinations from six half-sib sire families and several unrelated animals. Of these animals class I molecules were precipitated from cell lysates with the mAb B1.1G6 and HC10. Immunoprecipitates were analysed by SDS-PAGE and 1D-IEF. There was a good agreement between class I serological types and IEF banding patterns. We have identified three new class I specificities and subdivided the Bel 7 specificity. IEF has enabled us to make planned immunizations to produce antisera to the new specificities. New evidence for the expression of a second class I locus product in the Be7 haplotype has been found. |
| Databáze: | OpenAIRE |
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