Major Novel QTL for Resistance to Cassava Bacterial Blight Identified through a Multi-Environmental Analysis
| Autor: | Ruben Eduardo Mora Moreno, Fabio A. Gómez Cano, Camilo Ernesto López Carrascal, Jens Léon, Agim Ballvora, Boby Mathew, Johana Carolina Soto Sedano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Veterinary medicine Sequence analysis Population Plant Science genotype by environment interactions Quantitative trait locus Biology lcsh:Plant culture 01 natural sciences candidate defense-related genes 03 medical and health sciences Genotype lcsh:SB1-1110 education Gene Original Research QTL by environment interaction education.field_of_study Resistance (ecology) business.industry food and beverages Heritability WRKY protein domain Biotechnology 030104 developmental biology Xanthomonas axonopodis p.v manihotis business 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science, Vol 8 (2017) Frontiers in Plant Science |
| ISSN: | 1664-462X |
| DOI: | 10.3389/fpls.2017.01169 |
| Popis: | Cassava, Manihot esculenta Crantz, has been positioned as one of the most promising crops world-wide representing the staple security for more than one billion people mainly in poor countries. Cassava production is constantly threatened by several diseases, including cassava bacterial blight caused by Xanthomonas axonopodis pv. manihotis (Xam), it is the most destructive disease causing heavy yield losses. Here, we report the detection and localization on the genetic map of cassava QTL (Quantitative Trait Loci) conferring resistance to cassava bacterial blight (CBB). An F1 mapping population of 117 full sibs was tested for resistance to two Xam strains (Xam318 and Xam681) at two locations in Colombia: La Vega, Cundinamarca and Arauca. The evaluation was conducted in rainy and dry seasons and additional tests were carried out under controlled greenhouse conditions. The phenotypic evaluation of the response to Xam revealed continuous variation. Based on composite interval mapping analysis, 5 strain-specific QTL for resistance to Xam explaining between 15.8 and 22.1% of phenotypic variance, were detected and localized on a high resolution SNP-based genetic map of cassava. Four of them show stability among the two evaluated seasons. Genotype by environment analysis detected three QTL by environment interactions and the broad sense heritability for Xam318 and Xam681 were 20% and 53%, respectively. DNA sequence analysis of the QTL intervals revealed 29 candidate defense-related genes (CDRGs), and two of them contain domains related to plant immunity proteins, such as NB-ARC-LRR and WRKY. |
| Databáze: | OpenAIRE |
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