Differential expression of Low density lipoprotein Receptor-related Protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions

Autor: María M. Remedi, Mónica B. Gilardoni, Tristán Dellavedova, Mabel Oviedo, Laura Racca, J.P. Sarria, Mariana Domínguez, Claudia Gabriela Pellizas, Ana C. Donadio
Rok vydání: 2016
Předmět:
0301 basic medicine
PCA3
Male
Pathology
medicine.medical_specialty
CIENCIAS MÉDICAS Y DE LA SALUD
LRP-1
Prostatic Hyperplasia
Medicina Clínica
Matrix metalloproteinase
Prostatic Diseases
urologic and male genital diseases
Pathology and Forensic Medicine
Metastasis
03 medical and health sciences
Prostate cancer
Prostate
medicine
PROSTATE
Humans
IMMUNOHISTOCHEMISTRY
High-grade prostatic intraepithelial neoplasia
Aged
Aged
80 and over
Prostatic Intraepithelial Neoplasia
Chemistry
Prostatic Neoplasms
Cell Biology
Hyperplasia
Middle Aged
medicine.disease
CANCER
Prostatitis
030104 developmental biology
medicine.anatomical_structure
Matrix Metalloproteinase 9
lipids (amino acids
peptides
and proteins)
Medicina Critica y de Emergencia
MMP-9
ZYMOGRAPHY
Low Density Lipoprotein Receptor-Related Protein-1
Zdroj: Pathology, research and practice. 213(1)
ISSN: 1618-0631
Popis: Background Metalloproteinases (MMPs) are relevant modulators of inflammation, tumor microenvironment, cancer invasion and metastasis. They can be regulated by the Low density lipoprotein Receptor?related Protein 1 (LRP-1), a receptor reported to mediate the clearance of lipoproteins, extracellular matrix (ECM) macromolecules and proteinases. The aim of this study was to evaluate the expression of LRP-1, MMP-2 and MMP-9 across various grades of prostatic diseases as benign prostatic hyperplasia (BPH), BPH plus prostatitis (BPH + P), high grade prostatic intraepithelial neoplasia (HGPIN) and prostate cancer (PCa). Methods LRP-1 was analyzed using immunohistochemistry and MMPs proteolytic activity by zymography in prostate tissues with different prostatic diseases. Results LRP-1 was detected in epithelial cells in BPH (16/18), BPH + P (21/21) and HGPIN (6/6), with a staining intensity of 1+, 1+/2+ and 3+, respectively. In PCa, LRP-1 was absent in 19/27 samples while a low expression was observed in 8/27 biopsies. MMP-9 activity was higher and statistically significant in PCa than in BPH (p ≤ 0.01). Conclusion Considering that LRP-1, by mediating the clearance of MMPs, is involved in the regulation of ECM remodeling and cell migration, we conclude that a decreased expression of LRP-1 could be involved with the increasing activity of MMPs shown in cancers. Fil: Gilardoni, Mónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Remedi, Maria Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Oviedo, Mabel. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina Fil: Dellavedova, Tristán. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; Argentina Fil: Sarría, Juan P.. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; Argentina Fil: Racca, Laura. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; Argentina Fil: Dominguez, Mariana. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; Argentina Fil: Pellizas, Claudia Gabriela. Universidad Nacional de Córdoba; Argentina Fil: Donadio, Ana Carolina. Universidad Nacional de Córdoba; Argentina
Databáze: OpenAIRE
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