Neokestose suppresses the growth of human melanoma A2058 cells via inhibition of the nuclear factor-κB signaling pathway
| Autor: | Dey-Chyi Sheu, Shun‑Mei Lee, Jan Yi Chang, Jiann‑Shing Wu, Ming-Tse Lin, C. Will Chen |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Cell Survival Population Cell Gene Expression Apoptosis Biology Biochemistry Flow cytometry 03 medical and health sciences 0302 clinical medicine Cyclin D1 Transforming Growth Factor beta Cell Line Tumor Genetics medicine Humans Viability assay Phosphorylation education Melanoma Molecular Biology Cell Proliferation education.field_of_study medicine.diagnostic_test Cell growth Cell Cycle NF-kappa B Cell cycle 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cancer research Molecular Medicine Trisaccharides Signal Transduction |
| Zdroj: | Molecular Medicine Reports. 16:295-300 |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Neokestose has superior prebiotic effects compared with the commercial fructooligosaccharides (FOS). In addition, the branched structure of neokestose, a type of neo‑FOS, confers improved chemical stability compared with conventional FOS; therefore, the investigation of the branched structure by the present study may be of high biomedical value. The present study aimed to determine whether neokestose may suppress growth of the A2058 melanoma cell line. The cells were initially treated with neokestose; subsequently, in vitro cytotoxicity was assessed using MTT, and cell cycle progression and apoptosis were detected using flow cytometry. The protein expression levels of cyclin D1, phosphorylated (p)‑inhibitor of κB (IκB) and nuclear factor‑κB (NF‑κB) were determined using western blotting. Treatment with neokestose led to a dose‑dependent inhibition of cell viability. Flow cytometry data indicated that neokestose increased the sub‑G1 cell population, and induced early and late apoptosis. Western blot analysis revealed that neokestose treatment reduced the expression levels of p‑IκB and cyclin D1. These findings suggest that neokestose treatment may induce suppression of A2058 melanoma cell viability via inhibition of the NF‑κB pathway. The present findings support the requirement for further investigation into the potential use of neokestose as an additional or chemopreventive therapeutic agent for the treatment of melanoma. |
| Databáze: | OpenAIRE |
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