Grape seed proanthocyanidin extract alleviates high-fat diet induced testicular toxicity in rats
| Autor: | Yong Jun Bu, Zeng Li Yu, Er Hui Wang, Peng Wei Xu, Jin Yan Xi, Hai Yan Liang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Antioxidant General Chemical Engineering medicine.medical_treatment 02 engineering and technology 010402 general chemistry medicine.disease_cause 01 natural sciences chemistry.chemical_compound Internal medicine medicine chemistry.chemical_classification Spermatogenic Cell TUNEL assay food and beverages General Chemistry Glutathione 021001 nanoscience & nanotechnology 0104 chemical sciences Enzyme medicine.anatomical_structure Endocrinology chemistry Apoptosis 0210 nano-technology Oxidative stress Germ cell |
| Zdroj: | RSC Advances. 9:11842-11850 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c9ra01017c |
| Popis: | The present study aimed to investigate the protective effects of grape seed proanthocyanidin extract (GSPE) on high-fat diet (HFD) induced testicular damage, oxidative stress, and apoptotic germ cell death. Male rats (n = 40) were randomly divided into four groups: the control group (treated with physiological saline), HFD group, HFD + GSPE (100 mg kg−1) group and HFD + GSPE (300 mg kg−1) group. Compared with the HFD group the rats of the GSPE-treated group showed improved serum testosterone levels, sperm quality and histological appearance of the testis tissue. Significant elevation of antioxidant enzyme (SOD, GSH, and GSH-Px) activities and remarkable reduction in MDA were also observed by GSPE administration, indicating that GSPE can decrease testicular oxidative stress. Finally, a significant reduction in spermatogenic cell apoptosis was detected by TUNEL assay. In summary, these results indicated that GSPE can suppress testicular dysfunction and this effect may be attributed to its antioxidant and anti-apoptotic properties. The current study indicates that GSPE can be considered a promising candidate for use as a drug or a food supplement to alleviate HFD-induced testicular dysfunction. |
| Databáze: | OpenAIRE |
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