Potassium bisperoxo (1,10-phenanthroline) oxovanadate suppresses proliferation of hippocampal neuronal cell lines by increasing DNA methyltransferases
| Autor: | Jun-He Cui, Chunyang Zhang, Kerui Gong, Shaochun Yan, Xiaolei Liu, Guo Shao, Shuyuan Jiang, Xiaolu Zhang, Xiaoli Tian, Jie Yang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Methyltransferase hippocampal neurons viruses DNA methyltransferase lcsh:RC346-429 Flow cytometry 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience Gene expression potassium bisperoxo (1 10-phenanthroline) oxovanadate medicine Viability assay nerve regeneration 10-phenanthroline) oxovanadate p21 HT22 cell cell cycle immunoblotting DNA methylation neural regeneration lcsh:Neurology. Diseases of the nervous system medicine.diagnostic_test Cell growth Chemistry Cell cycle potassium bisperoxo (1 Molecular biology 030104 developmental biology Cell culture 030217 neurology & neurosurgery Research Article |
| Zdroj: | Neural Regeneration Research Neural Regeneration Research, Vol 14, Iss 5, Pp 826-833 (2019) |
| ISSN: | 1673-5374 |
| DOI: | 10.4103/1673-5374.249230 |
| Popis: | Bisperoxo (1,10-phenanthroline) oxovanadate (BpV) can reportedly block the cell cycle. The present study examined whether BpV alters gene expression by affecting DNA methyltransferases (DNMTs), which would impact the cell cycle. Immortalized mouse hippocampal neuronal precursor cells (HT22) were treated with 0.3 or 3 μM BpV. Proliferation, morphology, and viability of HT22 cells were detected with an IncuCyte real-time video imaging system or inverted microscope and 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium, respectively. mRNA and protein expression of DNMTs and p21 in HT22 cells was detected by real-time polymerase chain reaction and immunoblotting, respectively. In addition, DNMT activity was measured with an enzyme-linked immunosorbent assay. Effects of BpV on the cell cycle were analyzed using flow cytometry. Results demonstrated that treatment with 0.3 μM BpV did not affect cell proliferation, morphology, or viability; however, treatment with 3 μM BpV decreased cell viability, increased expression of both DNMT3B mRNA and protein, and inhibited the proliferation of HT22 cells; and 3 μM BpV also blocked the cell cycle and increased expression of the regulatory factor p21 by increasing DNMT expression in mouse hippocampal neurons. |
| Databáze: | OpenAIRE |
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