Bacterial Mucosa-associated Microbiome in Inflamed and Proximal Noninflamed Ileum of Patients With Crohn’s Disease
| Autor: | Arne K. Sandvik, Maya Olaisen, Elin Synnøve Røyset, Reidar Fossmark, Arnar Flatberg, Atle van Beelen Granlund, Tom C. Martinsen |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Crohn’s disease medicine.medical_specialty Adolescent Biopsy microbiome Inflammation Ileum Gastroenterology Pathogenesis Young Adult Ileocecal valve Crohn Disease Recurrence RNA Ribosomal 16S Internal medicine mucosal microbiota medicine Humans Immunology and Allergy Microbiome Intestinal Mucosa AcademicSubjects/MED00260 Crohn's disease medicine.diagnostic_test business.industry Mucous membrane Ileitis medicine.disease Gastrointestinal Microbiome medicine.anatomical_structure Case-Control Studies Female Leading Off medicine.symptom business |
| Zdroj: | Inflammatory Bowel Diseases |
| ISSN: | 1536-4844 1078-0998 |
| Popis: | Background Microbiota is most likely essential in the pathogenesis of Crohn’s disease (CD). Fecal diversion after ileocecal resection (ICR) protects against CD recurrence, whereas infusion of fecal content triggers inflammation. After ICR, the majority of patients experience endoscopic recurrence in the neoterminal ileum, and the ileal microbiome is of particular interest. We have assessed the mucosa-associated microbiome in the inflamed and noninflamed ileum in patients with CD. Methods Mucosa-associated microbiome was assessed by 16S rRNA sequencing of biopsies sampled 5 and 15 cm orally of the ileocecal valve or ileocolic anastomosis. Results Fifty-one CD patients and forty healthy controls (HCs) were included in the study. Twenty CD patients had terminal ileitis, with endoscopic inflammation at 5 cm, normal mucosa at 15 cm, and no history of upper CD involvement. Crohn’s disease patients (n = 51) had lower alpha diversity and separated clearly from HC on beta diversity plots. Twenty-three bacterial taxa were differentially represented in CD patients vs HC; among these, Tyzzerella 4 was profoundly overrepresented in CD. The microbiome in the inflamed and proximal noninflamed ileal mucosa did not differ according to alpha diversity or beta diversity. Additionally, no bacterial taxa were differentially represented. Conclusions The microbiome is similar in the inflamed and proximal noninflamed ileal mucosa within the same patients. Our results support the concept of CD-specific microbiota alterations and demonstrate that neither ileal sublocation nor endoscopic inflammation influence the mucosa-associated microbiome. The microbiota in the ileum is similar in inflamed and proximal noninflamed mucosa. Tyzzerella 4 is profoundly overrepresented in patients with Crohn’s disease. The microbiome disturbance is not limited to inflamed areas in patients with ileal Crohn’s disease. |
| Databáze: | OpenAIRE |
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