Valorization of kitchen biowaste for ethanol production via simultaneous saccharification and fermentation using co-cultures of the yeasts Saccharomyces cerevisiae and Pichia stipitis
| Autor: | Maria Alexandropoulou, Ioanna Ntaikou, Gerasimos Lyberatos, Georgia Antonopoulou, Nikolaos Menis |
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| Rok vydání: | 2019 |
| Předmět: |
Environmental Engineering
020209 energy Bioengineering Saccharomyces cerevisiae 02 engineering and technology Pichia chemistry.chemical_compound Hydrolysis Biotransformation Enzymatic hydrolysis 0202 electrical engineering electronic engineering information engineering Ethanol fuel Food science Pichia stipitis Waste Management and Disposal Ethanol biology Renewable Energy Sustainability and the Environment General Medicine biology.organism_classification Coculture Techniques bioethanol kitchen wastes yeast co-cultures enzymatic hydrolysis Refuse Disposal chemistry Biofuel Biofuels Fermentation |
| Popis: | The biotransformation of the pre-dried and shredded organic fraction of kitchen waste to ethanol was investigated, via co-cultures of the yeasts Saccharomyces cerevisiae and Pichia stipitis (Scheffersomyces stipitis). Preliminary experiments with synthetic media were performed, in order to investigate the effect of different operational parameters on the ethanol production efficiency of the co-culture. The control of the pH and the supplementation with organic nitrogen were shown to be key factors for the optimization of the process. Subsequently, the ethanol production efficiency from the waste was assessed via simultaneous saccharification and fermentation experiments. Different loadings of cellulolytic enzymes and mixtures of cellulolytic with amylolytic enzymatic blends were tested in order to enhance the substrate conversion efficiency. It was further shown that for solids loading up to 40% waste on dry mass basis, corresponding to 170g.L-1 initial concentration of carbohydrates, no substrate inhibition occurred, and ethanol concentration up to 45g.L-1 was achieved. The data set of the publication can be accessed at: http://doi.org/10.5281/zenodo.3906241 |
| Databáze: | OpenAIRE |
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