In vitro angiogenic performance and in vivo brain targeting of magnetized endothelial progenitor cells for neurorepair therapies
| Autor: | Cristina Boada, Anna Rosell, Elisa Carenza, Anna Laromaine, Joan Montaner, Anna Roig, Verónica Barceló, Anna Morancho, Lisa Levander |
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| Rok vydání: | 2014 |
| Předmět: |
Vascular Endothelial Growth Factor A
Angiogenesis medicine.medical_treatment Cell Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering Biology Fibroblast growth factor Ferric Compounds Endothelial progenitor cell Mice 03 medical and health sciences 0302 clinical medicine Microscopy Electron Transmission In vivo medicine Animals General Materials Science Progenitor cell Magnetite Nanoparticles 030304 developmental biology 0303 health sciences Staining and Labeling Stem Cells Growth factor Brain Endothelial Cells Cell Differentiation Anatomy Magnetic Resonance Imaging Cell biology Radiography medicine.anatomical_structure cardiovascular system Molecular Medicine Reactive Oxygen Species 030217 neurology & neurosurgery Ex vivo |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine. 10:225-234 |
| ISSN: | 1549-9634 |
| DOI: | 10.1016/j.nano.2013.06.005 |
| Popis: | Endothelial progenitor cells (EPCs) represent a promising approach for cell-based therapies to induce tissue repair; however, their effective delivery into the brain has remained a challenge. We loaded EPCs with superparamagnetic iron oxide nanoparticles (SPIONs), assessed their angiogenic potential and evaluated their guidance to the brain using an external magnet. SPIONs were stored in the cytoplasm within endosomes/lysosomes as observed by transmission electron microscopy (TEM) and could be visualized as hypointense signals by magnetic resonance imaging (MRI) T 2 -weighted images. In vitro SPION-loaded EPCs were fully functional, forming vessel-like structures in Matrigel®, and displayed enhanced migration and secretion of growth factors (VEGF and FGF), which was associated with a moderate increase in reactive oxygen species production. Furthermore, in vivo MRI of treated mice showed accumulated hypointense signals consistent with SPION-loaded EPCs engraftment. Thus, we demonstrate that loading EPCs with SPIONs represents a safe and effective strategy for precise cell guidance into specific brain areas. From the Clinical Editor This study investigates the potential role of endothelial progenitor cells in neuro-repair strategies of the central nervous system using SPION-loaded EPCs and magnetic guidance to the target organ. The authors demonstrate ex vivo cellular viability and maintained function following SPION load as well as successful guidance of the EPCs to the target site via MR imaging in a murine model. |
| Databáze: | OpenAIRE |
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