Analysis of Expressed Sequence Tag Loci on Wheat Chromosome Group 4
| Autor: | C. E. Bermudez-Kandianis, Nora L. V. Lapitan, Jose L. Gonzalez-Hernandez, Timothy J. Close, M. A. Rodriguez Milla, S. F. Kianian, Tatik Chikmawati, A. A. Mahmoud, J. Ramalingam, S Kim, M. S. Pathan, O. Feril, G S Surlan Momirovic, C Yaghoubian, R Skinner, Gerard R. Lazo, Xue-Feng Ma, D. W. Choi, Jorge Dubcovsky, Olin D. Anderson, Calvin O. Qualset, P Fang, A. M. Linkiewicz, V Nguyen, Miftahudin, Mark E. Sorrells, K. Ross, Lili Qi, K Chema, Muharrem Dilbirligi, J McAllister, Patrick E. McGuire, Venu Kalavacharla, Bikram S. Gill, Shiaoman Chao, James A. Anderson, Raymond D. Fenton, Henry T. Nguyen, E. Conley, Kulvinder S. Gill, L Haule, Khwaja Hossain, H Struxness, J Birkes, Eduard Akhunov, B. Echalier, Junhua Peng, J. P. Gustafson, Jamie Anne Layton, J. Dvořák |
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| Rok vydání: | 2004 |
| Předmět: |
0106 biological sciences
Chromosomal translocation Genes Plant 01 natural sciences Genome Chromosomes Plant Chromosomes Homology (biology) 03 medical and health sciences Gene Duplication Wheat Investigations Genetics Genomic library Triticeae Triticum Gene Library 030304 developmental biology Expressed Sequence Tags 0303 health sciences Expressed sequence tag biology Human Genome Chromosome Mapping food and beverages Chromosome Plant biology.organism_classification Genes Chromosome 3 Genome Plant Gene Deletion Biotechnology Developmental Biology 010606 plant biology & botany |
| Zdroj: | Genetics, vol 168, iss 2 |
| ISSN: | 1943-2631 |
| Popis: | A total of 1918 loci, detected by the hybridization of 938 expressed sequence tag unigenes (ESTs) from 26 Triticeae cDNA libraries, were mapped to wheat (Triticum aestivum L.) homoeologous group 4 chromosomes using a set of deletion, ditelosomic, and nulli-tetrasomic lines. The 1918 EST loci were not distributed uniformly among the three group 4 chromosomes; 41, 28, and 31% mapped to chromosomes 4A, 4B, and 4D, respectively. This pattern is in contrast to the cumulative results of EST mapping in all homoeologous groups, as reported elsewhere, that found the highest proportion of loci mapped to the B genome. Sixty-five percent of these 1918 loci mapped to the long arms of homoeologous group 4 chromosomes, while 35% mapped to the short arms. The distal regions of chromosome arms showed higher numbers of loci than the proximal regions, with the exception of 4DL. This study confirmed the complex structure of chromosome 4A that contains two reciprocal translocations and two inversions, previously identified. An additional inversion in the centromeric region of 4A was revealed. A consensus map for homoeologous group 4 was developed from 119 ESTs unique to group 4. Forty-nine percent of these ESTs were found to be homoologous to sequences on rice chromosome 3, 12% had matches with sequences on other rice chromosomes, and 39% had no matches with rice sequences at all. Limited homology (only 26 of the 119 consensus ESTs) was found between wheat ESTs on homoeologous group 4 and the Arabidopsis genome. Forty-two percent of the homoeologous group 4 ESTs could be classified into functional categories on the basis of blastX searches against all protein databases. |
| Databáze: | OpenAIRE |
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