Proteolytic activity of the proteasome is required for female insect reproduction
| Autor: | Yan-Yuan Bao, Lu-Yao Peng, Rui-Rui Yang, Lu Zhang, Yue-Lin Yao, Wei Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Proteasome Endopeptidase Complex Immunology Biology Halloween genes General Biochemistry Genetics and Molecular Biology Hemiptera Transcriptome reproduction 03 medical and health sciences Vitellogenin 0302 clinical medicine Transcription (biology) Animals Gene lcsh:QH301-705.5 030304 developmental biology 0303 health sciences Gene knockdown General Neuroscience Ovary RNA non-atpase regulatory subunit Cell biology Ecdysterone oocyte maturation Proteasome ovulation and hatching lcsh:Biology (General) Proteolysis proteolytic activity biology.protein Insect Proteins Female 030217 neurology & neurosurgery nilaparvata lugens |
| Zdroj: | Open Biology, Vol 11, Iss 2 (2021) |
| ISSN: | 2046-2441 |
| DOI: | 10.1098/rsob.200251 |
| Popis: | Non-ATPase regulatory subunits (Rpns) are components of the 26S proteasome involved in polyubiquitinated substrate recognition and deubiquitination in eukaryotes. Here, we identified 15 homologues sequences of Rpn and associated genes by searching the genome and transcriptome databases of the brown planthopper, Nilaparvata lugens , a hemipteran rice pest. Temporospatial analysis showed that NlRpn genes were significantly highly expressed in eggs and ovaries but were less-highly expressed in males. RNA interference-mediated depletion of NlRpn genes decreased the proteolytic activity of proteasome and impeded the transcription of lipase and vitellogenin genes in the fat bodies and ovaries in adult females, and reduced the triglyceride content in the ovaries. Decrease of the proteolytic activity of the proteasome via knockdown of NlRpn s also inhibited the transcription of halloween genes, including NlCYP307A2 , NlCYP306A2 and NlCYP314A1 , in the 20-hydroxyecdysone (20E) biosynthetic pathway in the ovaries, reduced 20E production in adult females, and impaired ovarian development and oocyte maturation, resulting in reduced fecundity. These novel findings indicate that the proteolytic activity of the proteasome is required for female reproductive processes in N. lugens , thus furthering our understanding of the reproductive and developmental strategies in insects. |
| Databáze: | OpenAIRE |
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