Floretlike Cells in In Situ and Prolapsed Orbital Fat
| Autor: | Shiquan Ren, Katayoon B. Ebrahimi, W. Richard Green |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Pathology medicine.medical_specialty Conjunctiva CD34 Antigens Differentiation Myelomonocytic Adipose tissue Antigens CD34 Immunoenzyme Techniques Masson's trichrome stain Antigens CD Prolapse In Situ Nick-End Labeling Orbital Diseases Humans Medicine Aged Retrospective Studies Aged 80 and over business.industry CD68 S100 Proteins Anatomy Middle Aged medicine.disease eye diseases Staining Ophthalmology medicine.anatomical_structure Adipose Tissue Female Pleomorphic lipoma business Biomarkers Immunostaining |
| Zdroj: | Ophthalmology. 114:2345-2349 |
| ISSN: | 0161-6420 |
| DOI: | 10.1016/j.ophtha.2007.06.007 |
| Popis: | Purpose Orbital pleomorphic lipoma has been rarely reported in the literature. Although floretlike cells are characteristic of pleomorphic lipoma, they are not pathognomonic. We reviewed cases of prolapsed orbital fat and exenteration specimens to determine the significance of presence of these cells in the diagnosis of orbital pleomorphic lipoma. Design Retrospective interventional case series with clinicopathologic correlation. Participants Seventy-two specimens of 45 patients with prolapsed orbital fat and 74 exenteration specimens as controls. Intervention Histologic review of the specimens including light microscopy, Masson trichrome staining, immunostaining for S100, CD34, CD68, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay, and transmission electron microscopy and review of clinical records and analysis of the data with generalized estimation equation. Main Outcome Measure Evidence of histologic abnormalities in histologic specimens and clinical and demographic data. Results Floretlike cells were present in 31 of 72 (43%) specimens of prolapsed orbital fat and in 12 of 74 (16%) orbital exenterations. Fewer than 6 florets were present in twenty 40× high-power fields in 15 (48%), 6 to 10 in 9 (29%), and >10 in 7 (23%) specimens. The florets stained positive for CD34 but not for S100 or CD68. TUNEL assay revealed significant nuclear pyknosis, and transmission electron microscopy disclosed spindle-shaped cells with abundant rough endoplasmic reticulum and no basement membrane. The mean age of patients with prolapsed orbital fat with florets was 67 years (range, 52–86). Of 31 samples, 29 (94%) were from males. Of 30 samples, 29 (97%) were located in the superotemporal conjunctiva; only one was located in the lower lid. There was significant association between the presence of florets and location of the prolapsed orbital fat ( P = 0.0013) and gender ( P = 0.0015). Conclusion Floretlike cells may be present in in situ and prolapsed orbital fat as a degenerative process. What some have called "orbital pleomorphic lipoma" is in fact only age-related orbital fat prolapse. |
| Databáze: | OpenAIRE |
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