Immunohistology of skin and oral biopsies in graft-versus-host disease after bone marrow transplantation and cytokine therapy
| Autor: | Ken Hamada, Tatsuo Yudate, Tadashi Tezuka, Hikikazu Yamada, Junichi Chihara, Keiji Maeda, Masaharu Matsukura, Kazuo Tubaki |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Keratinocytes
Pathology medicine.medical_specialty Cyclophosphamide medicine.medical_treatment Biopsy Immunology Graft vs Host Disease immune system diseases Granulocyte Colony-Stimulating Factor medicine Immunology and Allergy Humans Oral mucosa Bone Marrow Transplantation Chemotherapy integumentary system medicine.diagnostic_test business.industry Macrophage Colony-Stimulating Factor Mouth Mucosa Granulocyte-Macrophage Colony-Stimulating Factor Atopic dermatitis medicine.disease Granulocyte colony-stimulating factor surgical procedures operative Graft-versus-host disease medicine.anatomical_structure Acute Disease Chronic Disease Cytokines Bone marrow Epidermis business medicine.drug |
| Zdroj: | The Journal of allergy and clinical immunology. 100(6 Pt 2) |
| ISSN: | 0091-6749 |
| Popis: | Background: Early diagnosis of graft-versus-host-disease (GVHD) after bone marrow transplantation is often difficult, particularly when the patients are immunosuppressed by chemotherapy or irradiation. Objective: To investigate the influence of cytokines on skin lesions after bone marrow transplantation. Methods: Biopsy specimens of skin and oral mucosa were obtained from bone marrow transplant patients with GVHD and were subjected to histologic and immunohistochemical examination. Results: Administration of granulocyte-macrophage colony-stimulating factor caused atopic dermatitis–like lesions in two patients, who had infiltration of neutrophils, eosinophils, and lymphocytes around the hair follicles of the skin and no signs of GVHD in other organs. Only patients who were treated with cytokines developed acute GVHD. Immunohistochemical examination of skin biopsies from 18 patients with acute GVHD and 11 patients with chronic GVHD after cyclophosphamide administration or irradiation showed that the maculopapular skin lesions characteristic of acute GVHD contained infiltrates of CD4 + and CD8 + lymphocytes. There was also an increase in numbers of epidermal keratinocytes expressing intercellular adhesion molecule-I and HLA-DR antigens. Conclusion: These findings support the involvement of cytokines in GVHD and suggest that immunostaining of skin biopsies may be useful for the early diagnosis of this condition. (J Allergy Clin Immunol 1997;100:S73-6.) |
| Databáze: | OpenAIRE |
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