Norepinephrine Increases Glucose Transport in Brown Adipocytes via β3-Adrenoceptors through a cAMP, PKA, and PI3-Kinase-Dependent Pathway Stimulating Conventional and Novel PKCs
| Autor: | Tore Bengtsson, Ekaterina Chernogubova, Barbara Cannon |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Adrenergic receptor Glucose uptake Adrenergic Biology medicine.disease_cause Adenylyl cyclase Mice Norepinephrine Phosphatidylinositol 3-Kinases chemistry.chemical_compound Endocrinology Adipose Tissue Brown Internal medicine Brown adipose tissue Adipocytes Cyclic AMP medicine Animals Hypoglycemic Agents Insulin Cells Cultured Protein Kinase C Cholera toxin Isoproterenol Adrenergic beta-Agonists Cyclic AMP-Dependent Protein Kinases Protein Kinase A Inhibitor Glucose medicine.anatomical_structure chemistry Receptors Adrenergic beta-3 Female Signal transduction Adrenergic alpha-Agonists Signal Transduction |
| Zdroj: | Endocrinology. 145:269-280 |
| ISSN: | 1945-7170 0013-7227 |
| Popis: | To identify the signaling pathways that mediate the adrenergic stimulation of glucose uptake in brown adipose tissue, we used mouse brown adipocytes in culture. The endogenous adrenergic neurotransmitter norepinephrine (NE) induced 2-deoxy-D-glucose uptake 3-fold in a concentration-dependent manner (pEC50 approximately 6.5). The uptake was abolished by high doses of propranolol. The NE effect was mimicked by isoprenaline (pEC50 approximately 6.9), BRL 37344 (pEC50 approximately 8.6), CL 316243 (pEC50 approximately 9.7) and CGP 12177 (pEC50 approximately 7.3) and was thus mediated by beta3-adrenergic receptors. The NE-induced effect on 2-deoxy-D-glucose uptake was mediated by adenylyl cyclase and cAMP because responses were inhibited by the adenylyl cyclase inhibitor 2',5'-dideoxyadenosine and the protein kinase A inhibitor 4-cyano-3-methylisoquinoline. Cholera toxin and 8-bromoadenosine cAMP were both able to increase 2-deoxy-D-glucose uptake. Involvement of other adrenergic signaling pathways (alpha1-and alpha2-adrenergic receptors) were excluded. The phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002, abolished beta-adrenergic- or 8-bromoadenosine cAMP-stimulated 2-deoxy-D-glucose uptake, demonstrating that a cAMP-dependent PI3K-mediated pathway is positively connected to glucose uptake. Inhibition of the beta-adrenergically stimulated response with protein kinase C (PKC) inhibitors (Gö 6983, which inhibits (alpha, beta, gamma), (delta), and (zeta) isoforms and Ro-31-8220, which inhibits (alpha, beta1, beta2, gamma) and (epsilon) but not atypical isoforms) indicated that cAMP-mediated glucose uptake is stimulated via conventional and novel PKCs. These results demonstrate that adrenergic stimulation, through beta3-adrenergic receptors/cAMP/protein kinase A, recruits a PI3K pathway stimulating conventional and novel PKCs, which mediate glucose uptake in brown adipocytes. |
| Databáze: | OpenAIRE |
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