Methanogenesis and Salt Tolerance Genes of a Novel Halophilic Methanosarcinaceae Metagenome-Assembled Genome from a Former Solar Saltern
| Autor: | Clifton P. Bueno de Mesquita, Jinglie Zhou, Susannah G. Tringe, Susanna Theroux |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
16S
Salinity Methanogenesis archaea Euryarchaeota QH426-470 Genome Article RNA Ribosomal 16S Botany Genetics Ecosystem Phylogeny Genetics (clinical) Methanosarcinaceae Ribosomal salt tolerance biology Chemistry Methanolobus phylogenomics methanogenesis biology.organism_classification Methanogen Halophile Methanomethylovorans Wetlands anaerobic RNA Metagenome Methane Archaea |
| Zdroj: | Genes, Vol 12, Iss 1609, p 1609 (2021) Genes Volume 12 Issue 10 Genes, vol 12, iss 10 |
| ISSN: | 2073-4425 |
| Popis: | Anaerobic archaeal methanogens are key players in the global carbon cycle due to their role in the final stages of organic matter decomposition in anaerobic environments such as wetland sediments. Here we present the first draft metagenome-assembled genome (MAG) sequence of an unclassified Methanosarcinaceae methanogen phylogenetically placed adjacent to the Methanolobus and Methanomethylovorans genera that appears to be a distinct genus and species. The genome is derived from sediments of a hypersaline (97–148 ppt chloride) unrestored industrial saltern that has been observed to be a significant methane source. The source sediment is more saline than previous sources of Methanolobus and Methanomethylovorans. We propose a new genus name, Methanosalis, to house this genome, which we designate with the strain name SBSPR1A. The MAG was binned with CONCOCT and then improved via scaffold extension and reassembly. The genome contains pathways for methylotrophic methanogenesis from trimethylamine and dimethylamine, as well as genes for the synthesis and transport of compatible solutes. Some genes involved in acetoclastic and hydrogenotrophic methanogenesis are present, but those pathways appear incomplete in the genome. The MAG was more abundant in two former industrial salterns than in a nearby reference wetland and a restored wetland, both of which have much lower salinity levels, as well as significantly lower methane emissions than the salterns. |
| Databáze: | OpenAIRE |
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