Absence of Anti-Glomerular Basement Membrane Antibodies in 200 Patients With Systemic Lupus Erythematosus With or Without Lupus Nephritis: Results of the GOODLUPUS Study
| Autor: | Bernard Bonnotte, P. Orquevaux, Bach Nga Pham, Zahir Amoura, S. Revuz, Delphine Giusti, Laurent Arnaud, Moustapha Dramé, Gilles Blaison, Marcelle Tonye Libyh, Thierry Martin, Hanns-Martin Lorenz, Nadine Magy-Bertrand, Grace Stockton-Bliard, Reinhard E. Voll, Amélie Servettaz, Grégory Gatouillat, Jan Chrusciel, Nellie Bourse Chalvon, Jean-Loup Pennaforte, Andreas Schwarting, Thierry Tabary, Oliver Hinschberger |
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| Přispěvatelé: | Centre Hospitalier Universitaire de Reims (CHU Reims), Immuno-Régulation dans les Maladies Auto-Immunes Inflammatoires et le Cancer - EA 7509 (IRMAIC), Université de Reims Champagne-Ardenne (URCA) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Anti-Glomerular Basement Membrane Disease [SDV]Life Sciences [q-bio] Lupus nephritis Aucun urologic and male genital diseases Severity of Illness Index Gastroenterology anti-glomerular basement membrane antibodies 0302 clinical medicine systemic lupus erythematosus Lupus Erythematosus Systemic Immunology and Allergy 030212 general & internal medicine Original Research medicine.diagnostic_test biology anti-GBM glomerulonephritis Glomerular basement membrane IIf Middle Aged 3. Good health Titer medicine.anatomical_structure [SDV.IMM]Life Sciences [q-bio]/Immunology Female Antibody Vasculitis Adult lcsh:Immunologic diseases. Allergy medicine.medical_specialty Immunology 03 medical and health sciences Antigen Internal medicine anti-GBM antibodies medicine Humans Autoantibodies Retrospective Studies 030203 arthritis & rheumatology lupus nephritis business.industry Goodpasture disease medicine.disease Case-Control Studies Immunoassay biology.protein business lcsh:RC581-607 Biomarkers |
| Zdroj: | Frontiers in Immunology, Vol 11 (2020) Frontiers in Immunology Frontiers in Immunology, Frontiers, 2020, 11, ⟨10.3389/fimmu.2020.597863⟩ |
| ISSN: | 1664-3224 |
| Popis: | IntroductionAnti-glomerular basement membrane (GBM) antibodies are pathogenic antibodies first detected in renal-limited anti-GBM disease and in Goodpasture disease, the latter characterized by rapidly progressive crescentic glomerulonephritis combined with intra-alveolar hemorrhage. Studies have suggested that anti-GBM antibody positivity may be of interest in lupus nephritis (LN). Moreover, severe anti-GBM vasculitis cases in patients with systemic lupus erythematosus (SLE) have been described in the literature, but few studies have assessed the incidence of anti-GBM antibodies in SLE patients.ObjectiveThe main study objective was to determine if positive anti-GBM antibodies were present in the serum of SLE patients with or withoutproliferativerenal damage and compared to a healthy control group.MethodologyThis retrospective study was performed on SLE patients’ sera from a Franco-German European biobank, developed between 2011 and 2014, from 17 hospital centers in the Haut-Rhin region. Patients were selected according to their renal involvement, and matched by age and gender. The serum from healthy voluntary blood donors was also tested. Anti-GBM were screened by fluorescence enzyme immunoassay (FEIA), and then by indirect immunofluorescence (IIF) in case of low reactivity detection (titer >6 U/ml).ResultsThe cohort was composed of 100 SLE patients withproliferativeLN (27% with class III, 67% with class IV, and 6% with class V), compared to 100 SLE patients without LN and 100 controls. Patients were mostly Caucasian and met the ACR 1997 criteria and/or the SLICC 2012 criteria. Among the 300 tested sera, no significant levels of anti-GBM antibodies were detected (>10 U/ml) by the automated technique, three sera were found “ambivalent” (>7 U/ml): one in the SLE with LN group and two in the SLE without LN group. Subsequent IIF assays did not detect anti-GBM antibodies.ConclusionAnti-GBM antibodies were not detected in the serum of Caucasian patients with SLE, even in case of renal involvement, a situation favoring the antigenic exposure of glomerular basement membranes. Our results reaffirm the central role of anti-GBM antibodies as a specific diagnostic biomarker for Goodpasture vasculitis and therefore confirm that anti-GBM antibody must not be carried out in patients with SLE (with or without LN) in the absence of disease-suggestive symptoms. |
| Databáze: | OpenAIRE |
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