Recent insights into the structure, function, and regulation of the eukaryotic transfer RNA splicing endonuclease complex

Autor: Cassandra K. Hayne, Tanae A. Lewis, Robin E. Stanley
Rok vydání: 2022
Předmět:
Zdroj: WIREs RNA. 13
ISSN: 1757-7012
1757-7004
DOI: 10.1002/wrna.1717
Popis: The splicing of transfer RNA (tRNA) introns is a critical step of tRNA maturation, for intron-containing tRNAs. In eukaryotes, tRNA splicing is a multi-step process that relies on several RNA processing enzymes to facilitate intron removal and exon ligation. Splicing is initiated by the tRNA splicing endonuclease (TSEN) complex which catalyzes the excision of the intron through its two nuclease subunits. Mutations in all four subunits of the TSEN complex are linked to a family of neurodegenerative and neurodevelopmental diseases known as pontocerebellar hypoplasia (PCH). Recent studies provide molecular insights into the structure, function, and regulation of the eukaryotic TSEN complex and are beginning to illuminate how mutations in the TSEN complex lead to neurodegenerative disease. Using new advancements in the prediction of protein structure, we created a three-dimensional model of the human TSEN complex. We review functions of the TSEN complex beyond tRNA splicing by highlighting recently identified substrates of the eukaryotic TSEN complex and discuss mechanisms for the regulation of tRNA splicing, by enzymes that modify cleaved tRNA exons and introns. Finally, we review recent biochemical and animal models that have worked to address the mechanisms that drive PCH and synthesize these studies with previous studies to try to better understand PCH pathogenesis. This article is categorized under: RNA ProcessingtRNA Processing RNA in Disease and DevelopmentRNA in Disease RNA Interactions with Proteins and Other MoleculesProtein-RNA Recognition.
Databáze: OpenAIRE
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