Ankyrin Is An Intracellular Tether for TMC Mechanotransduction Channels

Autor: Siva A. Vanapalli, Sol Ah Lee, Yi-Quan Tang, William R Schafer, Hang Lu, Mizanur Rahman
Rok vydání: 2020
Předmět:
C. ELEGANS
0301 basic medicine
Mechanotransduction
Cellular
MECHANOSENSITIVITY
Ion Channels
0302 clinical medicine
tether
CIB
gating spring
Ankyrin
Mechanotransduction
HAIR-CELLS
Caenorhabditis elegans
chemistry.chemical_classification
biology
Chemistry
Cilium
General Neuroscience
Cell biology
Mechanoreceptor
medicine.anatomical_structure
C. elegans
MACHINERY
Hair cell
Life Sciences & Biomedicine
Mechanoreceptors
DEAFNESS
Intracellular
Ankyrins
hair cells
CALCIUM
Article
MECHANOELECTRICAL TRANSDUCTION
03 medical and health sciences
ankyrin
medicine
Animals
Caenorhabditis elegans Proteins
mechanotransduction channel
nose touch
Science & Technology
Mechanosensation
TMC
Neurosciences
biology.organism_classification
SENSATION
COMPONENT
030104 developmental biology
Neurosciences & Neurology
MEMBRANE
030217 neurology & neurosurgery
Zdroj: Neuron
ISSN: 0896-6273
Popis: Summary Mechanotransduction channels have been proposed as force sensors in various physiological processes, such as hearing and touch. In particular, TMC1 has been shown to constitute the pore of hair cell mechanotransduction channels, but little is known about how force is sensed by TMC channels. Here, we identify UNC-44/ankyrin as an essential component of the TMC-1 mechanotransduction channel complex in the sensory cilia of Caenorhabditis elegans mechanoreceptor neurons. Ankyrin binds indirectly to TMC-1 via evolutionarily conserved CIB proteins, which are required for TMC-1-mediated mechanosensation in C. elegans OLQ neurons and body wall muscles. Mechanosensory activity conferred by ectopically expressed TMCs in mechanoinsensitive neurons depends on both ankyrin and CIB proteins, indicating that the ankyrin-CIB subcomplex is required for TMC mechanosensitivity. Our work indicates that ankyrin is a long-sought intracellular tether that transmits force to TMC mechanotransduction channels.
Graphical Abstract
Highlights • TMC-1 functions as a mechanosensor in C. elegans neurons and muscles • UNC-44/ankyrin binds indirectly to TMC-1 via CALM-1 • CALM-1 and ankyrin are required for TMC-1-mediated mechanosensation • Ankyrin acts as an intracellular tether to confer mechanosensitivity to TMC channels
TMC channels are important for sensory transduction, notably as key components of the hair cell mechanotransducer. In this issue of Neuron, Tang et al. show that the mechanosensory activity of C. elegans TMCs requires the intracellular tether ankyrin, which interacts with TMC-1 through the adaptor protein CALM-1.
Databáze: OpenAIRE
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