Molecular genetic analysis of the major histocompatibility complex in an ELA typed horse family
| Autor: | M. Vaiman, Claudine Geffrotin, Daniel Cohen, Gérard Guérin, Patrick Chardon, Maud Bertaud |
|---|---|
| Přispěvatelé: | Unité de recherche Génétique Biochimique et Cytogénétique (LGBC), Institut National de la Recherche Agronomique (INRA), Laboratoire de radiobiologie et d'étude du génome (LREG), Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), ProdInra, Migration |
| Rok vydání: | 2009 |
| Předmět: |
Male
Genetic Linkage CONTROLE DE PARENTE EcoRI Genes MHC Class I [SDV.GEN] Life Sciences [q-bio]/Genetics HindIII IMMUNOLOGIE DNA sequencing Major Histocompatibility Complex 03 medical and health sciences 0302 clinical medicine Histocompatibility Antigens Complementary DNA MHC class I Genetics Animals Horses 030304 developmental biology Southern blot [SDV.GEN]Life Sciences [q-bio]/Genetics 0303 health sciences Polymorphism Genetic biology GENETIQUE ANIMALE General Medicine Molecular biology Restriction enzyme Blood Grouping and Crossmatching biology.protein Female POLYMORPHISME DE LONGUEUR DES FRAGMENTS DE RESTRICTION Animal Science and Zoology Restriction fragment length polymorphism Polymorphism Restriction Fragment Length 030215 immunology |
| Zdroj: | Animal Genetics Animal Genetics, 1987, 18, pp.323-336 Animal Genetics, Wiley-Blackwell, 1987, pp.323-336 |
| ISSN: | 1365-2052 0268-9146 |
| DOI: | 10.1111/j.1365-2052.1987.tb00776.x |
| Popis: | Restriction fragment length polymorphism was studied in an ELA typed horse family which included a stallion, a mare with two full-sibs, another mare with three full-sibs and, in addition, three paternal half-sibs. DNA samples from all individuals were investigated by Southern blot analysis using three restriction enzymes (EcoRI, HindIII or TaqI) and human cDNA class I, class II (DR beta) and class III (C4) probes. In addition, a genomic class II DQ alpha probe was used. Fragments hybridized with the various probes revealed the existence of DNA sequences homologous to HLA class I, DR beta, DQ alpha and C4 genes in the horse. Polymorphic fragments were found when DNA was hybridized with class I and class II probes irrespective of the enzyme used; but hybridization with the C4 probe did not reveal variability. All polymorphic fragments segregated according to the ELA serological specificities, thus indicating a close linkage between the different revealed subregions. Banding patterns suggest that the horse possesses about 20-30 class I genes, probably more than one DR beta and DQ alpha genes and possibly only one C4 gene. The high degree of polymorphism observed suggests that molecular DNA typing may represent a potentially powerful aid to decision in parentage control determination. |
| Databáze: | OpenAIRE |
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