Characterization of bovine coronavirus isolates/from eight different states in the USA
Autor: | Trisha R Maag, Kay L. Richardson, Sagar M. Goyal, Sanjay Kapil |
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Rok vydání: | 1999 |
Předmět: |
Antigenicity
Cattle Diseases Antibodies Viral medicine.disease_cause Microbiology Article Virus Mice Viral Proteins chemistry.chemical_compound Bovine coronavirus Tumor Cells Cultured medicine Animals Humans Coronavirus Coronavirus Bovine Antiserum General Veterinary biology Isoelectric focusing Hemagglutination Tests General Medicine Hemagglutination Inhibition Tests Virology United States Antigenic subtypes carbohydrates (lipids) chemistry Polyclonal antibodies biology.protein Cattle Hygromycin B Isoelectric Focusing Coronavirus Infections |
Zdroj: | Veterinary Microbiology |
ISSN: | 0378-1135 |
DOI: | 10.1016/s0378-1135(99)00042-5 |
Popis: | Bovine coronavirus isolates from eight different states of the USA were compared for their antigenic properties and susceptibility to hygromycin B. Antigenic differences were observed among the isolates in a one-way hemagglutination-inhibition (HI) test using a polyclonal antiserum against the Mebus bovine coronavirus isolate. Differences were observed on isoelectric focusing among viral proteins with isoelectric points between 4.45–4.65. Most of the BCV isolates were susceptible to hygromycin B (0.5 mM) whereas a few hygromycin B resistant isolates were also found. |
Databáze: | OpenAIRE |
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