'CLUPS': A New Culture Medium for the Axenic Growth of Entamoeba histolytica
| Autor: | Ofelia Monsivais-Diaz, Irene Meester, F. J. Guzmán De La Garza, Jesús Norberto Garza-González, L. H. De La Garza-Salinas, B. A. Barba-Dávila, Adriana Sampayo-Reyes, Javier Vargas-Villarreal, Magda Elizabeth Hernández-García, Francisco González-Salazar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
biology Article Subject 030231 tropical medicine Phosphoric Diester Hydrolases Raw beef Virulence biology.organism_classification medicine.disease In vitro Microbiology lcsh:Infectious and parasitic diseases 03 medical and health sciences Entamoeba histolytica 030104 developmental biology 0302 clinical medicine Infectious Diseases parasitic diseases medicine Parasitology lcsh:RC109-216 Amoebiasis Axenic Research Article Hepatic Abscesses |
| Zdroj: | Journal of Parasitology Research, Vol 2018 (2018) Journal of Parasitology Research |
| ISSN: | 2090-0023 |
| DOI: | 10.1155/2018/2796516 |
| Popis: | Amebiasis remains a major health problem in Mexico. Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. We present a new culture medium forEntamoeba histolyticawhich allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. The novel CLUPS medium is an improved version of the PEHPS medium, previously designed in our laboratory. The main difference is the substitution of raw beef liver in PEHPS by raw beef lung in the CLUPS medium. To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS),E. histolyticatrophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters.E. histolyticatrophozoites grew significantly better in CLUPS medium than in TYI-S-33. Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, trophozoites grown in any of the three tested media had similar potential to induce liver abscesses. |
| Databáze: | OpenAIRE |
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