Colorimetric aptasensor for on-site detection of oxytetracycline antibiotic in milk
| Autor: | Komal Birader, Yathirajarao Tammineni, Pankaj Kumar, Jeannie Alice Barla, Pankaj Suman, Shashidhar Reddy |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Circular dichroism
medicine.drug_class Aptamer Point-of-Care Systems Antibiotics Metal Nanoparticles Oxytetracycline Biosensing Techniques 01 natural sciences High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Limit of Detection medicine Animals Detection limit Chromatography 010401 analytical chemistry 04 agricultural and veterinary sciences General Medicine Aptamers Nucleotide 040401 food science 0104 chemical sciences Anti-Bacterial Agents Milk chemistry Gold Carrier Proteins Nitrocellulose Food Science medicine.drug Conjugate |
| Zdroj: | Food chemistry. 356 |
| ISSN: | 1873-7072 |
| Popis: | Oxytetracycline (OTC), one of the largely used antibiotic in veterinary practice has been banned due to its potential side effects. Development of a field applicable and affordable kit to detect OTC will help to eliminate such milk from human consumption. An aptamer has been designed (27 nt; Kd = 29.2 ± 19.4 nM) through rational truncation. OTC interacts with this aptamer in G rich regions as confirmed by molecular modelling and circular dichroism spectroscopy. To develop a lateral flow based aptasensor, OTC was conjugated with a 7 kDa carrier protein to immobilize onto the nitrocellulose membrane. Using 0.125 µM aptamer-gold conjugate, assay could visually detects upto 5 ng/mL of OTC in spiked milk within 10 mins [Limit of quantitation (LOQ)-0.254 ± 1.62 ng/mL; permissible limit 100 ng/mL]. It showed no cross reactivity with components of milk and data correlated with analysis done through HPLC. |
| Databáze: | OpenAIRE |
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