Antiviral effect of amiloride on replication of foot and mouth disease virus in cell culture
| Autor: | Huiyun Chang, Jun–jun Shao, Chang Yanyan, Gong Meijiao, Yongguang Zhang, Shi-fang Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
DNA Replication medicine.drug_class Cell Survival animal diseases viruses 030106 microbiology Picornaviridae Cell Culture Techniques Virus Replication Microbiology Antiviral Agents Virus Article Cell Line FMDV Amiloride 03 medical and health sciences Viral Proteins In vitro medicine Animals Humans RNA Messenger Antiviral activity Cytotoxicity biology virus diseases biology.organism_classification Virology 030104 developmental biology Infectious Diseases Viral replication Foot-and-Mouth Disease Virus Foot-and-Mouth Disease Foot-and-mouth disease virus Antiviral drug medicine.drug |
| Zdroj: | Microbial Pathogenesis |
| ISSN: | 1096-1208 0882-4010 |
| Popis: | Recently, amiloride was shown to potently suppress Coxsackievirus B3 (CVB3) replication. In the current study, we investigated whether amiloride could also exhibit antiviral activity against foot-and-mouth disease virus (FMDV), which belongs to the same family (Picornaviridae) as CVB3. We found that amiloride exerted antiviral activity in a dose-dependent manner against two strains of FMDV in IBRS-2 cells, with slight cytotoxicity at 1000 μM. Besides, amiloride did not inhibit the attachment and entry of FMDV in IBRS-2 cells, but prevented early viral replication. These data implied that amiloride could be a promising candidate for further research as a potential antiviral drug against FMDV infection. Highlights • This was the first study to demonstrate the antiviral activity of amiloride against FMDV. • Amiloride did not suppress the attachment and entry of FMDV into the cell. • The antiviral effect of amiloride occurred in the early phases of FMDV replication. |
| Databáze: | OpenAIRE |
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