A role for the RNA polymerase gene specificity factor sigma(54) in the uniform colony growth of uropathogenic escherichia coli
| Autor: | Amy Switzer, Lynn Burchell, Panagiotis Mitsidis, Teresa Thurston, Sivaramesh Wigneshweraraj |
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| Přispěvatelé: | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
EXPRESSION
Transcription Genetic ACID RESISTANCE Sigma Factor microcolonies Microbiology Bacterial Proteins 07 Agricultural and Veterinary Sciences Escherichia coli LOCUS Uropathogenic Escherichia coli TRANSCRIPTION FACTOR Molecular Biology RPON 11 Medical and Health Sciences sigma factors Adenosine Triphosphatases Science & Technology Escherichia coli Proteins ANTIBIOTIC-RESISTANCE DNA-Directed RNA Polymerases 06 Biological Sciences SIGMA-54 enzymes and coenzymes (carbohydrates) RNA polymerase sigma 54 BACTERIA health occupations ENTEROCYTE EFFACEMENT RNA UPEC transcription Life Sciences & Biomedicine |
| Popis: | The canonical function of a bacterial sigma (σ) factor is to determine the gene specificity of the RNA polymerase (RNAP). In several diverse bacterial species, the σ54 factor uniquely confers distinct functional and regulatory properties on the RNAP. A hallmark feature of the σ54-RNAP is the obligatory requirement for an activator ATPase to allow transcription initiation. Different activator ATPases couple diverse environmental cues to the σ54-RNAP to mediate adaptive changes in gene expression. Hence, the genes that rely upon σ54 for their transcription have a wide range of different functions suggesting that the repertoire of functions performed by genes, directly or indirectly affected by σ54, is not yet exhaustive. By comparing the growth patterns of prototypical enteropathogenic, uropathogenic, and nonpathogenic Escherichia coli strains devoid of σ54, we uncovered that the absence of σ54 results in two differently sized colonies that appear at different times specifically in the uropathogenic E. coli (UPEC) strain. Notably, UPEC bacteria devoid of individual activator ATPases of the σ54-RNAP do not phenocopy the σ54 mutant strain. Thus, it seems that σ54’s role as a determinant of uniform colony appearance in UPEC bacteria represents a putative non-canonical function of σ54 in regulating genetic information flow. |
| Databáze: | OpenAIRE |
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