Insulinotropic action of β-l -glucose pentaacetate
| Autor: | Aouatif Laghmich, Walter S. Zawalich, Abdullah Sener, María Luisa Villanueva-Peñacarrillo, Willy Malaisse, Dany Mercan, Karim Louchami, Marcel Kadiata, Carmen Sanchez-Soto, Concepción Viñambres, Isabel Valverde, André Herchuelz, Leonard Best, Marcia Hiriart, Hassan Jijakli, Elena Larrieta-Carasco, Olivier Scruel, Elizabeth Olivares |
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| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Physiology Endocrinology Diabetes and Metabolism medicine.medical_treatment Carbohydrate metabolism Biology Phosphatidylinositols Islets of Langerhans chemistry.chemical_compound Cations Physiology (medical) Internal medicine Cyclic AMP medicine Animals Insulin Phosphorylation Rats Wistar geography geography.geographical_feature_category Catabolism Hydrolysis Intracellular Membranes Metabolism Hydrogen-Ion Concentration Carbohydrate Islet Rats Electrophysiology Glutamine Glucose Endocrinology Biochemistry L-Glucose chemistry Calcium Adenylyl Cyclases |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 275:E993-E1006 |
| ISSN: | 1522-1555 0193-1849 |
| DOI: | 10.1152/ajpendo.1998.275.6.e993 |
| Popis: | The metabolism of β-l-glucose pentaacetate and its interference with the catabolism ofl-[U-14C]glutamine, [U-14C]palmitate,d-[U-14C]glucose, andd-[5-3H]glucose were examined in rat pancreatic islets. Likewise, attention was paid to the effects of this ester on the biosynthesis of islet peptides, the release of insulin from incubated or perifused islets, the functional behavior of individual B cells examined in a reverse hemolytic plaque assay of insulin secretion, adenylate cyclase activity in a membrane-enriched islet subcellular fraction, cAMP production by intact islets, tritiated inositol phosphate production by islets preincubated with myo-[2-3H]inositol, islet cell intracellular pH,86Rb and45Ca efflux from prelabeled perifused islets, and electrical activity in single isolated B cells. The results of these experiments were interpreted to indicate that the insulinotropic action of β-l-glucose pentaacetate is not attributable to any nutritional value of the ester but, instead, appears to result from a direct effect of the ester itself on a yet unidentified receptor system, resulting in a decrease in K+conductance, plasma membrane depolarization, and induction of electrical activity. |
| Databáze: | OpenAIRE |
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