Use of computerized motility analyzer for the evaluation of frozen-thawed ram spermatozoa

Autor:	Valcárcel A, Pérez L, Baldassarre H, Moses Df, de las Heras Ma
Rok vydání:	2009
Předmět:	Cryopreservation Male Spectrum analyzer Autoanalysis Hot Temperature Sheep Computers Urology Computer aid Motility Semen General Medicine Anatomy Biology Insemination Spermatozoa Lateral displacement Endocrinology Congelation Sperm Motility Animals Biomedical engineering
Zdroj:	Andrologia. 27:25-29
ISSN:	1439-0272 0303-4569
DOI:	10.1111/j.1439-0272.1995.tb02091.x
Popis:	Summary A computerized motility analyzer (CellTrak/STM) was calibrated for its use with ram semen. Adjustment of the several setup variables allowed an accurate measurement of kinetic parameters such as percentage of motile cells, straight velocity, curvilinear velocity, linearity and amplitude of lateral displacement of the head. All kinetic parameters, except the lateral displacement of the head, showed significant changes after freezing and thawing. The curvilinear velocity exhibited the least significative post-thaw decrease. The alterations in kinetic parameters provoked by freezing and thawing could account for the low success obtained with frozen semen by cervical insemination, as it is accepted that during the initial steps of fertilization high motility and linearity are required.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51e66052a46fe938edfda54e15a15b26 https://doi.org/10.1111/j.1439-0272.1995.tb02091.x Zobrazit plný text záznamu