New Method for DNA Microarrays Development: Applied to Human Platelet Antigens Polymorphisms
| Autor: | E. Vnuk, Yves Mérieux, Eliane Souteyrand, Vincent Dugas, Jean-Pierre Cloarec, Mehdi Jaber, Jean-Rene Martin, Jerome Broutin, Michel Cabrera, J.-C. Brès, D. Rigal |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Genotype
DNA Mutational Analysis Biomedical Engineering Polymerase Chain Reaction Polymorphism Single Nucleotide Sensitivity and Specificity law.invention law medicine Humans Antigens Human Platelet Molecular Biology Genotyping In Situ Hybridization Fluorescence Polymerase chain reaction Oligonucleotide Array Sequence Analysis biology Integrin beta3 Equipment Design medicine.disease Molecular biology Human platelet antigen Platelet transfusion refractoriness Equipment Failure Analysis genomic DNA Neonatal alloimmune thrombocytopenia biology.protein Feasibility Studies DNA microarray |
| Zdroj: | Biomedical Microdevices. 7:137-141 |
| ISSN: | 1572-8781 1387-2176 |
| Popis: | DNA microarrays are a powerful experimental tool for the detection of specific genomic sequences and are invaluable to a broad array of applications: clinical diagnosis, personalized medicine, drug research and development, gene therapy, food control technologies, and environmental sciences. Alloimmunization to human platelet antigens (HPAs) is commonly responsible for neonatal alloimmune thrombocytopenia, post-transfusional purpura and platelet transfusion refractoriness. Using DNA microarrays, we developed a diagnosis to type the biallelic HPA-1 platelet group. The region for the human genomic DNA sequence that contains the polymorphism responsible for HPA-1 alleles was amplified by polymerase chain reaction (PCR). The expected DNA fragments were hybridized on DNA microarrays, and the data were analyzed using specially developed software. Our initial results show that the two HPA-1 antigens polymorphisms containing a single base difference were detected using DNA microarrays. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink