Zinc supplementation within the reference ranges for zinc status in cattle improves sperm quality without modifying in vitro fertilization performance
| Autor: | Juan Patricio Anchordoquy, Luis Emilio Fazzio, Juan Mateo Anchordoquy, Raúl Martín Lizarraga, Esteban Martín Galarza, Nicolás Agustín Farnetano, Cecilia Cristina Furnus |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Cell Survival medicine.medical_treatment Fertilization in Vitro Biology Andrology 03 medical and health sciences Endocrinology Food Animals medicine Animals Blastocyst reproductive and urinary physiology 030304 developmental biology 0303 health sciences Pregnancy In vitro fertilisation urogenital system Artificial insemination Embryogenesis 0402 animal and dairy science Embryo 04 agricultural and veterinary sciences General Medicine medicine.disease Spermatozoa 040201 dairy & animal science Sperm Semen Analysis Zinc Pregnancy rate medicine.anatomical_structure Dietary Supplements embryonic structures Cattle Animal Science and Zoology |
| Zdroj: | Animal Reproduction Science. 221:106595 |
| ISSN: | 0378-4320 |
| DOI: | 10.1016/j.anireprosci.2020.106595 |
| Popis: | Zinc (Zn) has important functions in mammalian reproductive processes. In cattle, Zn status can be classified as deficient, marginal, and adequate, depending on the plasma Zn concentration. In addition, Zn deficiency can lead to reproductive failure. The aim of this study was to investigate the effect of maternal Zn status at the beginning of a fixed-time artificial insemination (FTAI) treatment regimen on pregnancy rate in cattle, and evaluate the effect of supplementing in vitro fertilization (IVF) medium with Zn concentrations within the reference range for Zn status on sperm quality and IVF performance. Pregnancy rates of animals with marginal and adequate Zn status did not differ, and there were no Zn-deficient animals detected. Supplementation of 0.8 μg/mL Zn to IVF medium enhanced progressive motility, sperm viability, functional sperm membrane integrity (HOST), acrosomal integrity and sperm-zona binding, without modifying pronuclear formation, or development of embryos to the cleavage or blastocyst stage after IVF. In conclusion, the present results indicate pregnancy rates are not associated with maternal Zn status at the beginning of the FTAI treatment regimen if Zn status is marginal or adequate. Furthermore, supplementation of IVF medium with Zn at concentrations which is considered adequate for Zn status in cattle led to improved sperm quality, without having effects on embryo development in cattle. |
| Databáze: | OpenAIRE |
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