Arachidonic acid production by the oleaginous fungus Mortierella alpina 1S-4 : A review
| Autor: | Jun Ogawa, Hiroshi Kikukawa, Sakayu Shimizu, Akinori Ando, Eiji Sakuradani |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030106 microbiology Mutant Review Article Fungus digestive system 03 medical and health sciences chemistry.chemical_compound Mortierella alpina Biosynthesis lcsh:Science (General) ComputingMethodologies_COMPUTERGRAPHICS chemistry.chemical_classification lcsh:R5-920 Multidisciplinary biology Fatty acid desaturase Fatty acid food and beverages biology.organism_classification eye diseases 030104 developmental biology Enzyme chemistry Biochemistry Arachidonic acid Molecular breeding biology.protein lipids (amino acids peptides and proteins) sense organs lcsh:Medicine (General) human activities lcsh:Q1-390 Polyunsaturated fatty acid |
| Zdroj: | Journal of Advanced Research, Vol 11, Iss, Pp 15-22 (2018) Journal of Advanced Research |
| ISSN: | 2090-1232 |
| Popis: | Graphical abstract The filamentous fungus Mortierella alpina 1S-4 is capable of accumulating a large amount of triacylglycerol containing C20 polyunsaturated fatty acids (PUFAs). Indeed, triacylglycerol production by M. alpina 1S-4 can reach 20 g/L of culture broth, and the critical cellular signaling and structural PUFA arachidonic acid (ARA) comprises 30%–70% of the total fatty acid. The demonstrated health benefits of functional PUFAs have in turn encouraged the search for rich sources of these compounds, including fungal strains showing enhanced production of specific PUFAs. Screening for mutants and targeted gene manipulation of M. alpina 1S-4 have elucidated the functions of various enzymes involved in PUFA biosynthesis and established lines with improved PUFA productivity. In some cases, these strains have been used for indistrial-scale production of PUFAs, including ARA. In this review, we described practical ARA production through mutant breeding, functional analyses of genes encoding enzymes involved in PUFA biosynthesis, and recent advances in the production of specific PUFAs through molecular breeding of M. alpina 1S-4. |
| Databáze: | OpenAIRE |
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