Direct noninvasive measurement and numerical modeling of depth-dependent strains in layered agarose constructs
| Autor: | van Cc René Donkelaar, Adam J. Griebel, Corey P. Neu, Tyler Novak, M Mehdi Khoshgoftar |
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| Přispěvatelé: | Orthopaedic Biomechanics |
| Rok vydání: | 2014 |
| Předmět: |
Cartilage
Articular Finite element method Materials science Heterogeneous strains Biomedical Engineering Biophysics Biocompatible Materials Functional tissue engineering Models Biological Article Sepharose chemistry.chemical_compound Chondrocytes Tissue engineering Regeneration Orthopedics and Sports Medicine Agarose scaffold Tissue Engineering Tissue Scaffolds Strain (chemistry) Regeneration (biology) Depth dependent Rehabilitation Biomaterial Imaging elastography Magnetic Resonance Imaging Reconstructive and regenerative medicine Radboud Institute for Health Sciences [Radboudumc 10] chemistry Agarose Stress Mechanical Biomedical engineering |
| Zdroj: | Journal of Biomechanics, 47, 2149-56 Journal of Biomechanics, 47(9), 2149-2156. Elsevier Journal of Biomechanics, 47, 9, pp. 2149-56 |
| ISSN: | 0021-9290 |
| Popis: | Contains fulltext : 137793.pdf (Publisher’s version ) (Closed access) Biomechanical factors play an important role in the growth, regulation, and maintenance of engineered biomaterials and tissues. While physical factors (e.g. applied mechanical strain) can accelerate regeneration, and knowledge of tissue properties often guide the design of custom materials with tailored functionality, the distribution of mechanical quantities (e.g. strain) throughout native and repair tissues is largely unknown. Here, we directly quantify distributions of strain using noninvasive magnetic resonance imaging (MRI) throughout layered agarose constructs, a model system for articular cartilage regeneration. Bulk mechanical testing, giving both instantaneous and equilibrium moduli, was incapable of differentiating between the layered constructs with defined amounts of 2% and 4% agarose. In contrast, MRI revealed complex distributions of strain, with strain transfer to softer (2%) agarose regions, resulting in amplified magnitudes. Comparative studies using finite element simulations and mixture (biphasic) theory confirmed strain distributions in the layered agarose. The results indicate that strain transfer to soft regions is possible in vivo as the biomaterial and tissue changes during regeneration and maturity. It is also possible to modulate locally the strain field that is applied to construct-embedded cells (e.g. chondrocytes) using stratified agarose constructs. |
| Databáze: | OpenAIRE |
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