Synthesis, Spectral Properties and DFT Calculations of new Ruthenium (II) Polypyridyl Complexes; DNA Binding Affinity and in Vitro Cytotoxicity Activity
| Autor: | Nagababu Penumaka, Venkat Reddy Putta, K Nagasuryaprasad, Ravi Kumar Vuradi, Nagamani Chintakuntla, Ramesh Kumar Chitumalla, Satyanarayana Sirasani, Rajender Reddy Mallepally, Madhuri P, Joonkyung Jang, Satyanarayana Singh S |
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| Rok vydání: | 2017 |
| Předmět: |
Staphylococcus aureus
Sociology and Political Science Cell Survival Pyridines Stereochemistry Phenanthroline Clinical Biochemistry chemistry.chemical_element Antineoplastic Agents 010402 general chemistry 01 natural sciences Biochemistry Medicinal chemistry Ruthenium chemistry.chemical_compound Coordination Complexes Bromide Escherichia coli Organometallic Compounds Tumor Cells Cultured Humans Spectroscopy Binding Sites Quenching (fluorescence) 010405 organic chemistry DNA Ligand (biochemistry) Intercalating Agents Anti-Bacterial Agents 0104 chemical sciences Molecular Docking Simulation Clinical Psychology chemistry Density functional theory Cyclic voltammetry Reactive Oxygen Species Law Social Sciences (miscellaneous) |
| Zdroj: | Journal of Fluorescence. 27:1513-1530 |
| ISSN: | 1573-4994 1053-0509 |
| Popis: | In this paper a novel ligand debip (2–(4–N,N–diethylbenzenamine)1H–imidazo[4,5–f] [1, 10]phenanthroline) and its Ru(II) polypyridyl complexes [Ru(L)2(debip)]2+, (L = phen (1), bpy (2) and dmb (3)) have been synthesized and characterized by spectroscopic techniques. The DNA binding studies for all these complexes were examined by absorption, emission, quenching studies, viscosity measurements and cyclic voltammetry. The light switching properties of complexes 1–3 have been evaluated. Molecular docking, Density Functional Theory (DFT) and time dependent DFT calculations were performed. The Ru(II) complexes exhibited efficient photocleavage activity against pBR322 DNA upon irradiation and exhibited good antimicrobial activity. Also investigated 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay, lactate dehydrogenase (LDH) release assay and reactive oxygen species (ROS) against selected cancer cell lines (HeLa, PC3, Lancap, MCF-7 and MD-MBA 231). |
| Databáze: | OpenAIRE |
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