FN3 linked nanobubbles as a targeted contrast agent for US imaging of cancer-associated human PD-L1

Autor: Uday S. Kumar, Arutselvan Natarajan, Tarik F. Massoud, Ramasamy Paulmurugan
Rok vydání: 2022
Předmět:
Zdroj: Journal of Controlled Release. 346:317-327
ISSN: 0168-3659
DOI: 10.1016/j.jconrel.2022.04.030
Popis: PD-L1 (programmed death-ligand 1) targeted therapies may be useful for several cancers. The use of non-invasive diagnostic and prognostic molecular imaging platforms could improve clinical assessment of PD-L1 tumor status during these therapies. Contrast enhanced ultrasound molecular imaging (CE-USMI) techniques may offer versatile and cost-effective ways to detect and quantify the expression levels of cellular targets in vivo. However, conventional use of microbubbles as a blood pool contrast agent for CE-USMI is limited to accessing intravascular biomarkers rather than reflecting the tumor molecular status. Using a microfluidic based reconstruction process we therefore developed ultra-stable nanobubbles (NBs) as a contrast agent for molecular imaging of vascular and extravascular cell surface markers. We then functionalized these NBs by covalently linking to nanobody (FN3
Databáze: OpenAIRE
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