The detection of great crested newts year round via environmental DNA analysis
| Autor: | Ben C. Maddison, Kevin C. Gough, David S. Gardner, Claire A. Baker, Helen C. Rees |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine animal structures Zoology lcsh:Medicine Environment 010603 evolutionary biology 01 natural sciences General Biochemistry Genetics and Molecular Biology Sampling season 03 medical and health sciences Animals Environmental DNA Great crested newt Ponds Survey lcsh:Science (General) lcsh:QH301-705.5 Great crested newts biology Ecology Pond lcsh:R DNA General Medicine biology.organism_classification Triturus Research Note 030104 developmental biology England lcsh:Biology (General) Environmental DNA (eDNA) embryonic structures Seasons lcsh:Q1-390 |
| Zdroj: | BMC Research Notes, Vol 10, Iss 1, Pp 1-4 (2017) BMC Research Notes |
| ISSN: | 1756-0500 |
| DOI: | 10.1186/s13104-017-2657-y |
| Popis: | Objective Analysis of environmental DNA (eDNA) is a method that has been used for the detection of various species within water bodies. The great crested newt (Triturus cristatus) has a short eDNA survey season (mid-April to June). Here we investigate whether this season could be extended into other months using the current methodology as stipulated by Natural England. Results Here we present data to show that in monthly water samples taken from two ponds (March 2014–February 2015) we were able to detect great crested newt DNA in all months in at least one of the ponds. Similar levels of great crested newt eDNA (i.e. highly positive identification) were detected through the months of March–August, suggesting it may be possible to extend the current survey window. In order to determine how applicable these observations are for ponds throughout the rest of the UK, further work in multiple other ponds over multiple seasons is suggested. Nevertheless, the current work clearly demonstrates, in two ponds, the efficacy and reproducibility of eDNA detection for determining the presence of great crested newts. Electronic supplementary material The online version of this article (doi:10.1186/s13104-017-2657-y) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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