lncRNA ATXN8OS promotes breast cancer by sequestering miR‑204
| Autor: | Liying Lin, Shunliang Yang, Huayu Cai, Weizhen Wu, Lingfeng Zhu, Jianming Tan, Jinquan Cai, Zhen Deng |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cancer Research proliferation Cell Breast Neoplasms Biology Biochemistry 03 medical and health sciences breast cancer 0302 clinical medicine Cell Line Tumor Genetics medicine Humans Neoplasm Invasiveness Molecular Biology Cell Proliferation long non-coding RNA Oncogene Cell growth Competing endogenous RNA Cancer Oncogenes Articles Cell cycle invasion medicine.disease Molecular medicine Gene Expression Regulation Neoplastic MicroRNAs tumorigenesis 030104 developmental biology medicine.anatomical_structure Oncology Cell culture 030220 oncology & carcinogenesis MCF-7 Cells Cancer research Molecular Medicine RNA Long Noncoding microRNA-204 |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Breast cancer (BC) is a common malignancy among women and the leading cause of female cancer mortality worldwide. In recent years, increasing evidence has shown that long non-coding RNAs (lncRNAs) can act as competing endogenous RNAs (ceRNAs) in human cancer and that they are involved in many biological processes, including proliferation, migration, apoptosis and invasion. In the present study, the biological function and molecular mechanism of ataxin 8 opposite strand (ATXN8OS) in BC tissue and cell lines were investigated. It was found that ATXN8OS was markedly up-regulated in BC tissue and cell lines, and that its level of overexpression was inversely linked with the overall survival rate of patients with BC. Knockdown of ATXN8OS inhibited proliferation, viability and invasion in the human MCF7 and MDA-MB-231 BC cell lines. In addition, microRNA-204 (miR-204) was negatively associated with the expression of ATXN8OS in BC tissues and cell lines. A luciferase assay demonstrated a direct binding site for miR-204 within ATXN8OS, and inhibition of miR-204 stimulated the tumour-promoting effect of ATXN8OS on BC cells. In conclusion, the present study suggested that ATXN8OS acts as a tumour promoter by sequestering miR-204 during the development of BC, therefore providing a mechanistic insight which may facilitate the diagnosis and treatment of BC. |
| Databáze: | OpenAIRE |
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