Ebselen Preserves Tissue-Engineered Cell Sheets and their Stem Cells in Hypothermic Conditions
| Autor: | Kohji Nishida, Ryosuke Katori, Yuki Kobayashi, Eiji Kobayashi, Ryuhei Hayashi |
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| Rok vydání: | 2016 |
| Předmět: |
Azoles
0301 basic medicine Cell Survival NF-E2-Related Factor 2 Hypothermia Isoindoles Regenerative medicine Article Cornea 03 medical and health sciences chemistry.chemical_compound Adenosine Triphosphate Cryoprotective Agents 0302 clinical medicine Organoselenium Compounds medicine Animals Humans Viability assay Progenitor cell Multidisciplinary Tissue Engineering Ebselen Stem Cells Epithelium Rats Cell biology Transplantation 030104 developmental biology medicine.anatomical_structure chemistry 030221 ophthalmology & optometry Tissue Preservation Rats Transgenic Stem cell Reactive Oxygen Species |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Clinical trials have been performed using autologous tissue-engineered epithelial cell sheets for corneal regenerative medicine. To improve stem cell-based therapy for convenient clinical practice, new techniques are required for preserving reconstructed tissues and their stem/progenitor cells until they are ready for use. In the present study, we screened potential preservative agents and developed a novel medium for preserving the cell sheets and their stem/progenitor cells; the effects were evaluated with a luciferase-based viability assay. Nrf2 activators, specifically ebselen, could maintain high ATP levels during preservation. Ebselen also showed a strong influence on maintenance of the viability, morphology, and stem cell function of the cell sheets preserved under hypothermia by protecting them from reactive oxygen species-induced damage. Furthermore, ebselen drastically improved the preservation performance of human cornea tissues and their stem cells. Therefore, ebselen shows good potential as a useful preservation agent in regenerative medicine as well as in cornea transplantation. |
| Databáze: | OpenAIRE |
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