Lectin spatial immunolocalization during in vitro capacitation in Tursiops truncatus spermatozoa
| Autor: | María Del Carmen Fuentes-Albero, Paula Sáez-Espinosa, María José Gómez-Torres, Daniel García-Párraga, Laura Robles-Gómez, Alejandro Romero, Natalia Huerta-Retamal |
|---|---|
| Přispěvatelé: | Universidad de Alicante. Departamento de Biotecnología, Grupo de Inmunología, Biología Celular y del Desarrollo |
| Rok vydání: | 2020 |
| Předmět: |
Peanut agglutinin
endocrine system Acrosome reaction Population Biología Celular 03 medical and health sciences 0302 clinical medicine Agglutinin Capacitation medicine Zona pellucida education education.field_of_study 030219 obstetrics & reproductive medicine General Veterinary biology urogenital system Bottlenose dolphin 0402 animal and dairy science Lectin Sperm capacitation 04 agricultural and veterinary sciences 040201 dairy & animal science Wheat germ agglutinin Cell biology medicine.anatomical_structure biology.protein Animal Science and Zoology |
| Zdroj: | Animal Reproduction, Volume: 17, Issue: 1, Article number: e20190083, Published: 14 FEB 2020 RUA. Repositorio Institucional de la Universidad de Alicante Universidad de Alicante (UA) |
| ISSN: | 1984-3143 |
| DOI: | 10.21451/1984-3143-ar2019-0083 |
| Popis: | Spermatozoa interactions with the female reproductive tract and oocyte are regulated by surface molecules such as glycocalyx. The capacitation process comprises molecular and structural modifications which increase zona pellucida binding affinity. Lectins allowed us to describe glycocalyx changes during maturation, capacitation and acrosome reaction. This study had as its aim to identify lectin binding patterns using four lectins with different carbohydrate affinity in bottlenose dolphin (Tursiops truncatus) spermatozoa both before and after in vitro capacitation. Two semen samples from the same dolphin obtained on consecutive days were used, with four different lectin binding patterns becoming visible in both samples before and after capacitation. A highly stained equatorial segment with prolongations at the edges appeared as the most frequent pattern with Wheat germ agglutinin (WGA) in uncapacitated spermatozoa. However, it was homogeneously distributed over the acrosomal region after capacitation. Instead, the use of Peanut agglutinin (PNA) resulted in most spermatozoa showing high labelling in the acrosomal periphery region before capacitation and a homogeneous staining in the acrosomal region within the population of capacitated spermatozoa. Nevertheless, the most representative patterns with Concavalin A (ConA) and Aleuria aurantia agglutinin (AAA) lectins did not change before and after capacitation, labelling the acrosomal region periphery. These findings could contribute to the understanding of the reproductive biology of cetaceans and the improvement of sperm selection techniques. Cátedra Human Fertility of University of Alicante and VIGROB-186. |
| Databáze: | OpenAIRE |
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