Monoclonal antibody (SBU-1 and SBU-3) identification of cells dissociated from the sheep placentomal trophoblast
| Autor: | M R Brandon, M M Ralph, K J Gogolin-Ewens, C.S. Lee |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Histology medicine.drug_class Placenta Gestational Age Biology Endometrium Monoclonal antibody Giant Cells Fetal membrane Internal medicine Keratin medicine Animals reproductive and urinary physiology chemistry.chemical_classification Sheep Antibodies Monoclonal Trophoblast Fibroblasts Molecular biology Trophoblasts medicine.anatomical_structure Endocrinology chemistry Cytoplasm embryonic structures Keratins Chorionic villi Anatomy |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 38:649-652 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/38.5.1692053 |
| Popis: | We studied the localization of alpha-keratin in the sheep placenta using an alpha-keratin-specific monoclonal antibody (MAb) SBU-1, and examined the feasibility of using this MAb as a marker for determining the purity of isolated uninucleate cells from the placentomal trophoblast. At about 30-50 days of gestation the placentomal and interplacentomal uninucleate cells and some binucleate cells were stained by SBU-1, whereas only the apical region of the syncytial cytoplasm was stained with this MAb. Other cells stained included the uterine and endometrial glandular epithelial cells and fibroblast-like cells in the endometrium and chorionic villi. At about 100-130 days of gestation only the trophoblast uninucleate cells were stained by SBU-1. Approximately 60% of cells isolated from placentomes at 100-130 days of gestation were stained by SBU-1, and they had similar morphological features to the trophoblast uninucleate cells. The number of binucleate cells present was confirmed by their affinity for MAb SBU-3. These results show that MAb SBU-1 is an excellent marker for trophoblast uninucleate cells from placenta of sheep at the later stages of pregnancy. |
| Databáze: | OpenAIRE |
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