Metabolite Imaging Using Matrix-Enhanced Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (ME-SALDI-MS)
| Autor: | Lin He, Yu M. Chiu, Qiang Liu, Coral Pagan-Miranda, Yongsheng Xiao |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
MALDI imaging
Brain Chemistry Diagnostic Imaging Matrix-assisted laser desorption electrospray ionization Chromatography Surface-assisted laser desorption/ionization Chemistry Myocardium Analytical chemistry Sensitivity and Specificity Mass spectrometry imaging Sample preparation in mass spectrometry Atmospheric-pressure laser ionization Surface-enhanced laser desorption/ionization Matrix-assisted laser desorption/ionization Mice Structural Biology Cerebellum Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Animals Female Dimyristoylphosphatidylcholine Cerebrum Spectroscopy |
| Zdroj: | Journal of the American Society for Mass Spectrometry. (1):80-88 |
| ISSN: | 1044-0305 |
| DOI: | 10.1016/j.jasms.2008.09.011 |
| Popis: | We describe here the use of a hybrid ionization approach, matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry (ME-SALDI-MS) in bioimaging. ME-SALDI combines the strengths of traditional matrix-assisted laser desorption/ionization (MALDI) and SALDI and enables successful MS imaging of low-mass species with improved detection sensitivity. Using 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) as the MS standard, MS performances of MALDI, SALDI, and ME-SALDI are systematically compared. The analyte desorption and ionization mechanism in ME-SALDI is qualitatively speculated based on the observation of significantly reduced matrix background and improved survival yields of molecular ions. Improvements in detection sensitivity of low-mass species using ME-SALDI over MALDI in imaging are demonstrated with mouse heart and brain tissues. |
| Databáze: | OpenAIRE |
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