'Wax On, Wax Off': In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy

Autor: Mark J. Tobin, Alan D. Payne, Mark J. Hackett, Annaleise R. Klein, Fatima Naim, Mark R. Gibberd, Lilian M. V. P. Sanglard, Karina Khambatta, Georgina Sauzier, Jitraporn Vongsvivut, Ashley Hollings
Rok vydání: 2021
Předmět:
0106 biological sciences
In situ
reflectance
General Chemical Engineering
Science
General Physics and Astronomy
Medicine (miscellaneous)
01 natural sciences
Biochemistry
Genetics and Molecular Biology (miscellaneous)
epicuticular wax
Epicuticular wax
Plant Epidermis
03 medical and health sciences
Stress
Physiological
Microscopy
General Materials Science
Fourier transform infrared spectroscopy
Plant Physiological Phenomena
Research Articles
030304 developmental biology
Plant Diseases
0303 health sciences
Wax
plant physiology
Chemistry
fungi
General Engineering
Plant physiology
food and beverages
Plant disease
Plant Leaves
13. Climate action
visual_art
Waxes
infrared
visual_art.visual_art_medium
Biophysics
Microscopy
Electron
Scanning
microscopy
Preclinical imaging
010606 plant biology & botany
Research Article
Zdroj: Advanced Science
Advanced Science, Vol 8, Iss 19, Pp n/a-n/a (2021)
ISSN: 2198-3844
Popis: Analysis of the epicuticular wax layer on the surface of plant leaves can provide a unique window into plant physiology and responses to environmental stimuli. Well‐established analytical methodologies can quantify epicuticular wax composition, yet few methods are capable of imaging wax distribution in situ or in vivo. Here, the first report of Fourier transform infrared (FTIR) reflectance spectroscopic imaging as a non‐destructive, in situ, method to investigate variation in epicuticular wax distribution at 25 µm spatial resolution is presented. The authors demonstrate in vivo imaging of alterations in epicuticular waxes during leaf development and in situ imaging during plant disease or exposure to environmental stressors. It is envisaged that this new analytical capability will enable in vivo studies of plants to provide insights into how the physiology of plants and crops respond to environmental stresses such as disease, soil contamination, drought, soil acidity, and climate change.
Fourier transform infrared (FTIR) reflectance spectroscopic imaging has been used to image epicuticular waxes on the surface of plant leaves, in living plants, to observe how plant physiology changes in response to environmental stressors or disease.
Databáze: OpenAIRE
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