Effects of peptides derived from dietary proteins on mucus secretion in rat jejunum
| Autor: | Jean Claustre, Jean Alain Chayvialle, Gerard Jourdan, Férial Toumi, Aurélien Trompette, Pascale Plaisancié, Henri Guignard |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty animal structures Protein Hydrolysates 030309 nutrition & dietetics Physiology Peptide In Vitro Techniques Biology Hydrolysate Jejunum 03 medical and health sciences Physiology (medical) Casein Internal medicine medicine Animals Secretion Rats Wistar 030304 developmental biology 2. Zero hunger chemistry.chemical_classification Lactalbumin 0303 health sciences Hepatology Hydrolysis Gastroenterology Caseins Mucus Peptide Fragments Small intestine Rats Endocrinology medicine.anatomical_structure chemistry Biochemistry Dietary Proteins Endorphins |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 283:G521-G528 |
| ISSN: | 1522-1547 0193-1857 |
| Popis: | The hypothesis that dietary proteins or their hydrolysates may regulate intestinal mucin discharge was investigated in the isolated vascularly perfused rat jejunum using an enzyme-linked immunosorbent assay for rat intestinal mucins. On luminal administration, casein hydrolysate [0.05–5% (wt/vol)] stimulated mucin secretion in rat jejunum (maximal response at 417% of controls). Lactalbumin hydrolysate (5%) also evoked mucin discharge. In contrast, casein, and a mixture of amino acids was without effect. Chicken egg albumin and its hydrolysate or meat hydrolysate also did not modify mucin release. Interestingly, casein hydrolysate-induced mucin secretion was abolished by intra-arterial TTX or naloxone (an opioid antagonist). β-Casomorphin-7, an opioid peptide released from β-casein on milk ingestion, induced a strong mucin secretion (response at 563% of controls) that was inhibited by naloxone. Intra-arterial β-casomorphin-7 also markedly increased mucin secretion (410% of controls). In conclusion, two enzymatic milk protein hydrolysates (casein and lactalbumin hydrolysates) and β-casomorphin-7, specifically, induced mucin release in rat jejunum. The casein hydrolysate-induced mucin secretion is triggered by a neural pathway and mediated by opioid receptor activation. |
| Databáze: | OpenAIRE |
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