ATP-binding cassette transporter A1 is significantly involved in the intestinal absorption of alpha- and gamma-tocopherol but not in that of retinyl palmitate in mice
| Autor: | Doriane Trompier, Emmanuelle Reboul, Alexis Klein, Jean-François Landrier, Giovanna Chimini, Myriam Moussa, Patrick Borel |
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| Přispěvatelé: | Nutrition humaine et lipides : Biodisponibilité, métabolisme et régulation, Université de la Méditerranée - Aix-Marseille 2-Institut National de la Recherche Agronomique (INRA)-Université de Provence - Aix-Marseille 1-IFR125-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biochimie [Purpan], Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut Fédératif de Biologie (IFB) - Hôpital Purpan, Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse]-Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse], Departement of Mathematics, University of California [Irvine] (UCI), University of California-University of California, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Institut de physiopathologie humaine de Marseille (IPHM), Université de la Méditerranée - Aix-Marseille 2-Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Université de la Méditerranée - Aix-Marseille 2-Institut National de la Recherche Agronomique ( INRA ) -Université de Provence - Aix-Marseille 1-IFR125-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Institut de biologie et chimie des protéines [Lyon] ( IBCP ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Université Paul Sabatier - Toulouse 3 ( UPS ) -CHU Toulouse [Toulouse]-Hôpital Purpan - Institut Fédératif de Biologie (IFB), University of California [Irvine] ( UCI ), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Centre d'Immunologie de Marseille - Luminy ( CIML ), Aix Marseille Université ( AMU ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Retinyl Esters
030309 nutrition & dietetics medicine.medical_treatment alpha-Tocopherol Medicine (miscellaneous) MESH : Nutritional Status Intestinal absorption Transgenic chemistry.chemical_compound Mice MESH: Vitamin E [ SDV.IMM ] Life Sciences [q-bio]/Immunology Vitamin E MESH: Animals Tocopherol Vitamin A Chromatography High Pressure Liquid MESH: Vitamin A 0303 health sciences Chromatography Nutrition and Dietetics Retinol MESH: Nutritional Status MESH : Mice Transgenic MESH : gamma-Tocopherol MESH: gamma-Tocopherol Cholesterol MESH: alpha-Tocopherol Mice Inbred DBA MESH : alpha-Tocopherol High Pressure Liquid MESH : Cholesterol HDL MESH: ATP-Binding Cassette Transporters [SDV.IMM]Life Sciences [q-bio]/Immunology lipids (amino acids peptides and proteins) MESH: Caco-2 Cells Diterpenes MESH: Cholesterol HDL ATP Binding Cassette Transporter 1 Vitamin medicine.medical_specialty HDL MESH : Mice Inbred DBA Active MESH: Mice Transgenic MESH: Intestinal Absorption gamma-Tocopherol Biological Transport Active Nutritional Status Mice Transgenic MESH : Vitamin A MESH : Chromatography High Pressure Liquid 03 medical and health sciences MESH : Vitamin E Internal medicine Retinyl palmitate MESH : Mice medicine Animals Humans Inbred DBA MESH : ATP-Binding Cassette Transporters MESH : Biological Transport Active MESH: Chromatography High Pressure Liquid MESH: Mice 030304 developmental biology MESH: Humans MESH: Mice Inbred DBA MESH : Humans Cholesterol HDL MESH : Intestinal Absorption MESH : Caco-2 Cells Biological Transport Endocrinology chemistry Intestinal Absorption MESH: Biological Transport Active ATP-Binding Cassette Transporters MESH : Animals Caco-2 Cells |
| Zdroj: | American Journal of Clinical Nutrition American Journal of Clinical Nutrition, American Society for Nutrition, 2009, 89 (1), pp.177-84. ⟨10.3945/ajcn.2008.26559⟩ The American Journal of Clinical Nutrition The American Journal of Clinical Nutrition, 2009, 89 (1), pp.177-84. ⟨10.3945/ajcn.2008.26559⟩ American Journal of Clinical Nutrition, American Society for Nutrition, 2009, 89 (1), pp.177-84. 〈10.3945/ajcn.2008.26559〉 |
| ISSN: | 0002-9165 1938-3207 |
| DOI: | 10.3945/ajcn.2008.26559⟩ |
| Popis: | International audience; BACKGROUND: It has long been assumed that newly absorbed vitamin A and E enter the body only via enterocyte-produced chylomicrons. However, recent results in cell cultures have shown that a fraction of alpha-tocopherol is secreted with intestinal HDL. OBJECTIVES: The aims of this study were to identify this transporter and to assess whether it is significantly implicated in the in vivo intestinal absorption of the 2 main dietary forms of vitamin E (ie, alpha- and gamma-tocopherol) and in that of retinyl palmitate (vitamin A). DESIGN: Having performed preliminary experiments in the Caco-2 cell model, we compared fasting and postprandial plasma concentrations of vitamins A and E in mice deficient in ATP-binding cassette A1 (ABCA1) transporter and in wild-type mice. RESULTS: A substantial efflux of alpha- and gamma-tocopherol, but not of retinyl esters, was induced by the presence of apolipoprotein A-I at the basolateral side of Caco-2 monolayers. The efflux of alpha- and gamma-tocopherol was also impaired by glyburide and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. The postprandial response of plasma gamma-tocopherol was 4-fold lower in ABCA1(-/-) mice (P = 0.025) than in wild-type mice, whereas no significant difference was observed for retinyl esters. Fasting plasma alpha-tocopherol, but not vitamin A, concentrations were lower in mice bearing the genetic deletion. CONCLUSIONS: ABCA1 is the transporter responsible for the in vivo secretion of alpha- and gamma-tocopherol with intestinal HDL, and this pathway is significantly implicated in the intestinal absorption and plasma status of vitamin E but not of vitamin A. |
| Databáze: | OpenAIRE |
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