Determination of Antipyrine and Metabolites in Plasma of a Patient With Mild Renal Failure
| Autor: | W. C. Ping, Silvia Regina Cavani Jorge Santos, Vera Lucia Lanchote |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Adult
Metabolic Clearance Rate Stereochemistry Sodium Metabolite chemistry.chemical_element Phenazone chemistry.chemical_compound Pharmacokinetics Reference Values Edaravone Blood plasma medicine Humans Pharmacology (medical) Chromatography High Pressure Liquid Pharmacology Chloroform Chromatography Anti-Inflammatory Agents Non-Steroidal chemistry Hypertension Kidney Failure Chronic Female Sodium acetate Quantitative analysis (chemistry) Antipyrine medicine.drug |
| Zdroj: | Therapeutic Drug Monitoring. 19:705-710 |
| ISSN: | 0163-4356 |
| DOI: | 10.1097/00007691-199712000-00017 |
| Popis: | A method has been developed for the simultaneous determination of antipyrine and its three major metabolites in plasma of patients with renal failure. Plasma samples (500 microl) were hydrolyzed with beta-glucuronidase/aryl sulphatase. The compounds, after addition of sodium chloride, were extracted with chloroform:ethanol (90:10, v/v) in acidic medium. Chromatographic conditions comprise a C18 column, a mobile phase with 30% methanol and 70% 0.25N sodium acetate buffer (pH 5.0), a total run time of 10 minutes, and ultraviolet absorbance detection at 254 nm. Confidence limits showed 0.5 to 40.0 microg/ml(-1) linearity (r2 = 0.999); 0.1 microg/ml(-1) HMA, 0.05 microg/ml(-1) antipyrine and NORA, and 0.5 microg/ml(-1) OHA sensitivity and absolute recovery >95%. Interprecision and intraprecision expressed as coefficient of variation were |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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